FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477858
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22340.46750289835055603No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13100.27414001649025443No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11900.24902795391099447No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT11750.245888946088587No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGACCCCCATG11590.24254067107801902No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11100.2322865788581545No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10760.22517149446069754No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8740.1828995224522766No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8710.1822717208877951No Hit
GCCTAACAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT8350.17473810211401714No Hit
CCCTTATCTCCACCTCTAAGGGAAACTCATCCTCAGGCCATGCAGTGGAA7920.16573961302311566No Hit
GTATCGGGAGGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACGG7830.16385620832967115No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT7660.16029866613094268No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT7500.15695039112037468No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT7380.15443918486244868No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7320.15318358173348567No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA7160.1498353067229177No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC6950.1454406957715472No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATATTCG6850.1433480238899422No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG6750.1412553520083372No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT6740.1410460848201767No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA6430.13455880198720122No Hit
CTTATAAGGCCTTTCTAAGTAAACAATATCAGAACCTTTACCCCGTTGCT6400.13393100042271971No Hit
CTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTT6400.13393100042271971No Hit
ATGTAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC6360.13309393167007774No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGGAAGTATTTGGTAACATT6240.13058272541215174No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6210.1299549238476702No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6090.1274437175897442No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCC6080.12723445040158374No Hit
CTGTTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5990.12535104570813924No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTATAAGGCC5940.12430470976733674No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG5940.12430470976733674No Hit
ATATTGTACAAAGAATCAAATTATGTTTTCGGAAACTTCCTGTAAACAGG5900.12346764101469475No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5890.12325837382653425No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5850.12242130507389225No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC5850.12242130507389225No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5840.12221203788573175No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCCATGAA5820.12179350350941073No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC5780.12095643475676875No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5710.11949156443964526No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA5690.11907303006332426No Hit
GTATGGATGGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC5620.11760815974620076No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5580.11677109099355876No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATATTAGGAAA5540.11593402224091676No Hit
ATCTAAGGGGAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA5530.11572475505275624No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA5520.11551548786459577No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCTTCCTGC5520.11551548786459577No Hit
GGGTAAAGGTTCTGATATTGTTTACTTAGAAAGGCCTTATAAGTTGGCGA5500.11509695348827477No Hit
ATTCTGAGCAGGGCTCACTGTCCCTGAACTGGAGCCACCAGCAGGAAAGT5500.11509695348827477No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAG5470.11446915192379326No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5450.11405061754747225No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5440.11384135035931175No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5430.11363208317115127No Hit
GCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA5410.11321354879483027No Hit
GGAACATATTGTACAAAGAATCAAATTATGTTTTCGGAAACTTCCTGTAA5380.11258574723034877No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT5330.11153941128954628No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5310.11112087691322527No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5280.11049307534874377No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5240.10965600659610178No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAACTCCAGATTGGGACCT5230.10944673940794128No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5230.10944673940794128No Hit
GTATTCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTATA5210.10902820503162028No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5190.10860967065529926No Hit
ATCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGC5170.10819113627897826No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGACCGAGATCTTCTGCGAC5160.10798186909081776No Hit
ATGTATACTTGCGGGATAGCACGACAGAATTGTCAGTCCCGATAAGTTTT5150.10777260190265728No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAATTGC5120.10714480033817578No Hit
GTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCTG5040.10547066283289179No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5020.10505212845657078No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAGAAGACTA5020.10505212845657078No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5010.10484286126841028No Hit
ATGTGGTATTGGGGACCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4960.10379652532760779No Hit
GAATACTAACATTGAGATTCCCGAGACCGAGATCTTCTGCGACGCGGCGA4910.10275018938680529No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTCTATTCTTCTACGG4910.10275018938680529No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4890.10233165501048427No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG4850.10149458625784229No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4830.10107605188152129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)