FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352542
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT14970.4246302568204639No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14260.40449081244220547No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAATTTCAT12850.3644955778318612No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA12470.3537167202773003No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA11890.3372647797992863No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT11850.3361301632145957No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10120.28705799592672643No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA9420.26720220569464065No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9070.25727431057859773No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG8650.24536083643934622No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8580.24337525741613764No Hit
GTATAGATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8520.24167333253910175No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8290.23514928717713068No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT8240.2337310164462674No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT8210.23288005400774942No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7720.21898100084528938No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC7710.21869734669911672No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC7480.21217330133714565No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT7450.21132233889862767No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7020.1991252106132035No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6970.19770693988234023No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC6940.1968559774438223No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG6940.1968559774438223No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG6660.18891366135098797No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC6570.18636077403543408No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT6310.17898576623494505No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6290.17841845794259975No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6270.17785114965025445No Hit
CATCTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6240.17700018721173646No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5950.16877421697272948No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG5640.1599809384413772No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT5460.15487516381026942No Hit
GCTTTGCTCCAGACCGGCTGCGAGCAAAGCAAGCGGCTAGGAGTTCCGCA5430.15402420137175146No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5200.1475001560097804No Hit
CTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT5070.14381265210953587No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5040.14296168967101794No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4850.1375722608937375No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA4850.1375722608937375No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4810.13643764430904687No Hit
CCTAATACATAGATCATTAACCTAGTCTCCTCCCCCAGTTCCTCCCAGTC4760.13501937357818358No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4710.13360110284732032No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAGATC4640.13161552382411174No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4610.13076456138559378No Hit
GTATCATCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA4460.12650974919300395No Hit
CTCCGAAAGAGACCAAATATTCAAGTACCGTCTCTCTTCCAAAAGTAAGG4460.12650974919300395No Hit
GGGTAAAGGTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA4430.125658786754486No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4400.12480782431596803No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4340.1231058994389321No Hit
CAAAACAACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGG4270.1211203204157235No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA4250.12055301212337821No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT4220.11970204968486024No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAGTACCTATC4170.11828377895399698No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC4130.11714916236930635No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA3990.11317800432288919No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAT3980.11289435017671652No Hit
GTGTCTATGAGATCTCTGATGGAAGGAAAGAAGTCAGAAGGCAAAAACGA3930.11147607944585326No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATCTAT3930.11147607944585326No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT3900.1106251170073353No Hit
CTTATAAAGAGTTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT3890.11034146286116263No Hit
GCTCATCGGTACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT3830.10863953798412672No Hit
GCTGTGCTCCAGACCGGCTGCGAGCAAAGCAAGCGGCTAGGAGTTCCGCA3830.10863953798412672No Hit
GTCTATGAGATCTCTGATGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3800.10778857554560875No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3800.10778857554560875No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3780.10722126725326343No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT3780.10722126725326343No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA3700.10495203408388219No Hit
TTCAAGTACCGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA3690.10466837993770954No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC3650.10353376335301893No Hit
CCCATAGGTATTTTGCGAAAGCCCATGACGAGGGGATGGGAATACAGGTG3620.10268280091450097No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG3610.10239914676832831No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG3600.10211549262215565No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT3570.10126453018363771No Hit
CCCGTGTGTCGTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC3560.10098087603746504No Hit
ATTTACAAGAGGACATTATTGATAGGTGTCAACAATTTGTGGGCCCTCTC3560.10098087603746504No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA3560.10098087603746504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)