FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352542
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14230.40363985000368746No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT13320.37782732270197594No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA12520.35513499100816354No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT12200.34605805833063863No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA12160.344923441745948No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10450.2964185827504241No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAATTTCAT10120.28705799592672643No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9570.2714570178872304No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA9330.26464931837908673No Hit
GTATAGATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8990.25500507740921646No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT8240.2337310164462674No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7970.2260723544996057No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7910.2243704296225698No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7880.22351946718405183No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG7560.21444253450652687No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT7510.2130242637756636No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT7470.211889647190973No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG7420.21047137646010972No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC7350.20848579743690115No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG7290.20678387255986522No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7010.19884155646703086No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6950.19713963158999498No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6710.19033193208185126No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC6380.18097134525815364No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6300.1787021120887724No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6110.17331268331149197No Hit
CATCTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5800.16451940478013968No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT5760.16338478819544905No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5740.16281747990310375No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5690.16139920917224046No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5540.15714439697965066No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5530.156860742833478No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5290.15005304332533428No Hit
GCTTTGCTCCAGACCGGCTGCGAGCAAAGCAAGCGGCTAGGAGTTCCGCA5160.14636553942508976No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT5040.14296168967101794No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4950.14040880235546405No Hit
CTTATAAAGAGTTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT4860.13785591503991013No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA4830.13700495260139217No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4800.13615399016287422No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4760.13501937357818358No Hit
CTCCGAAAGAGACCAAATATTCAAGTACCGTCTCTCTTCCAAAAGTAAGG4760.13501937357818358No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAGATC4760.13501937357818358No Hit
GTATCATCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA4700.13331744870114767No Hit
GGGTAAAGGTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA4700.13331744870114767No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA4690.133033794554975No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA4570.12962994480090315No Hit
CTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT4490.1273607116315219No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4360.12367320773127742No Hit
TTCAAGTACCGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4300.12197128285424147No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAT4240.12026935797720556No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4200.11913474139251493No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC4160.11800012480782432No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT4100.11629819993078838No Hit
CCTAATACATAGATCATTAACCTAGTCTCCTCCCCCAGTTCCTCCCAGTC4100.11629819993078838No Hit
CCCGTGTGTCGTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4070.11544723749227044No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4010.11374531261523449No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC3890.11034146286116263No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT3830.10863953798412672No Hit
GTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA3810.1080722296917814No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3750.10637030481474548No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA3750.10637030481474548No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAGTACCTATC3740.10608665066857283No Hit
ATTTACAAGAGGACATTATTGATAGGTGTCAACAATTTGTGGGCCCTCTC3700.10495203408388219No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG3680.1043847257915369No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC3670.10410107164536425No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATCTAT3610.10239914676832831No Hit
CAAAACAACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGG3600.10211549262215565No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT3590.101831838475983No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA3580.10154818432981036No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC3570.10126453018363771No Hit
GTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGTACAATATGAT3570.10126453018363771No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGCCTGT3570.10126453018363771No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3560.10098087603746504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)