FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438916
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30610.6973999580785389No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG23750.5411058152357171No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT20240.46113607159456477No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC16720.3809384939259448No Hit
CCTATTAATAATGTCTTCTTGTAAATGAATGTTAGGAAAGGAAGGAGTTT15940.36316743978346655No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT14080.32079031067447983No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTACC13180.3002852482023895No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC12530.28547603641699093No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA12430.28319769614231427No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT12220.27841318156549316No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAC11920.27157816074146307No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT11740.267477148247045No Hit
CATTTAAACCCTAACAAAACGAAAAGATGGGGTTATTCCCTAAACTTCAT11680.266110144082239No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA11230.2558576128461938No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC10670.24309890730800426No Hit
GTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTACCTGATCTGG10580.24104840106079525No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9890.22532785316552598No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCAAAACCAGACATTATT9650.2198598365063019No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9410.21439181984707778No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGCATTAGAGGACAAACGGGCAAC9120.20778463305051537No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9050.20618979485824165No Hit
GGGTATACATTTAAACCCTAACAAAACGAAAAGATGGGGTTATTCCCTAA8930.20345578652862964No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA8870.2020887823638236No Hit
ATATAAGACCTTGGGCAAGATCTGATGGGCATTCACGGTGGTCTCCATGC8710.19844343792434085No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG8270.1884187407157634No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT7930.1806723837818626No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA7550.1720146907380911No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7080.1613064914471106No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6930.15788898103509555No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6850.1560663088153542No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTACA6820.15538280673295116No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAACATTCATTTACAAG6820.15538280673295116No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6780.1544714706230805No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAT6770.15424363659561283No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT6760.15401580256814515No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6740.15356013451320982No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6570.14968695604625942No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA6450.14695294771664735No Hit
CAAATTGTTGACACCTATTAATAATGTCTTCTTGTAAATGAAAAGAGAAG6290.14330760327716463No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5960.13578908037073154No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5810.13237156995871646No Hit
ATACTAACATGGGTATAAAGTTCAGGCAACTATTGTGGTTTCATATATCT5760.1312323998213781No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAGTCACCCGTG5730.13054889773897513No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5730.13054889773897513No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG5660.12895405954670142No Hit
GAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTACCTGATCT5630.1282705574642984No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTTGAAG5630.1282705574642984No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT5570.12690355329949238No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5560.12667571927202473No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5390.12280254080507431No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC5360.12211903872267132No Hit
ATGTTAATACTAACATGGGTATAAAGTTCAGGCAACTATTGTGGTTTCAT5220.11892936233812393No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTA5210.11870152831065625No Hit
CATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAA5190.11824586025572091No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5140.11710669011838257No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA5140.11710669011838257No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAGTACCTATC5040.11482834984370585No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA5020.11437268178877051No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA4960.1130056776239645No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC4920.11209434151409382No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4880.11118300540422313No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4840.11027166929435245No Hit
GGTATAAAGTTCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4840.11027166929435245No Hit
GTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAAT4820.10981600123941711No Hit
CTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT4810.10958816721194943No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA4740.10799332901967575No Hit
CCATTTACACAATGTGGATATCCTGCCTTGATGCCTTTGTATGCATGTAT4630.10548715471753137No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4610.10503148666259604No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4550.10366448249779002No Hit
CTCCTAGACACCGCCTCAGCTCTGTTTCGAGATGCCTTAGAATCTCCTGA4540.10343664847032234No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTTGA4520.102980980415387No Hit
TGTTAATACTAACATGGGTATAAAGTTCAGGCAACTATTGTGGTTTCATA4510.10275314638791933No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4510.10275314638791933No Hit
GAATTAAACCTTATTATCCAGATCAGGTAGTTAATCATTACTTCAAAACC4410.10047480611324264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)