FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438916
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30570.6964886219686683No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG23570.537004802741299No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT22170.5051080388958251No Hit
CCTATTAATAATGTCTTCTTGTAAATGAATGTTAGGAAAGGAAGGAGTTT15330.34926956410793863No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC14130.3219294808118182No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT13190.30051308222985723No Hit
GTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTACCTGATCTGG12840.29253889126848875No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT12510.2850203683620556No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTACC12150.2768183433732195No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT11990.27317299893373675No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAC11660.26565447602730363No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC11580.26383180380756227No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC11110.2531236045165818No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA11110.2531236045165818No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10890.248111255912293No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA10760.2451494135552133No Hit
CATTTAAACCCTAACAAAACGAAAAGATGGGGTTATTCCCTAAACTTCAT10510.23945356286852157No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10390.2367195545389095No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9460.21553098998441617No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCAAAACCAGACATTATT9400.21416398581961013No Hit
GGGTATACATTTAAACCCTAACAAAACGAAAAGATGGGGTTATTCCCTAA8930.20345578652862964No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG8470.19297542126511677No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGCATTAGAGGACAAACGGGCAAC8400.19138058307284309No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA8190.186596068496022No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA8150.18568473238615132No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT7970.18158371989173328No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTACA7610.17338169490289712No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA7210.16426833380419034No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG7020.1599394872823046No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAT6890.15697764492522487No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAACATTCATTTACAAG6790.15469930465054818No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6780.1544714706230805No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT6650.15150962826600078No Hit
ATATAAGACCTTGGGCAAGATCTGATGGGCATTCACGGTGGTCTCCATGC6620.15082612618359778No Hit
GTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAAT6560.14945912201879175No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6520.14854778590892107No Hit
GAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTACCTGATCT6420.14626944563424438No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAGTCACCCGTG6410.1460416116067767No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5830.13282723801365182No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG5760.1312323998213781No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5740.13077673176644278No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5670.1291818935741691No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5640.12849839149176606No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA5560.12667571927202473No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5550.12644788524455705No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT5520.12576438316215405No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5480.12485304705228334No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA5330.12143553664026831No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAGTACCTATC5270.12006853247546227No Hit
CTCCTAGACACCGCCTCAGCTCTGTTTCGAGATGCCTTAGAATCTCCTGA5270.12006853247546227No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTA5260.11984069844799461No Hit
ATACTAACATGGGTATAAAGTTCAGGCAACTATTGTGGTTTCATATATCT5240.11938503039305927No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5150.11733452414585024No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTTGAAG5150.11733452414585024No Hit
ATGTTAATACTAACATGGGTATAAAGTTCAGGCAACTATTGTGGTTTCAT5120.11665102206344723No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA5090.11596751998104421No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA5050.11505618387117353No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4990.11368917970636752No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4970.11323351165143215No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4940.11255000956902916No Hit
CATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAA4890.1114108394316908No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC4880.11118300540422313No Hit
GTTTTGAAGTAATGATTAACTACCTGATCTGGATAATAAGGTTTAATTCC4810.10958816721194943No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4800.10936033318448178No Hit
GAATTAAACCTTATTATCCAGATCAGGTAGTTAATCATTACTTCAAAACC4660.10617065679993438No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC4650.10594282277246671No Hit
CTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT4570.10412015055272536No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTTGA4510.10275314638791933No Hit
ATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAG4490.10229747833298398No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAACATTCA4430.10093047416817798No Hit
CCATTTACACAATGTGGATATCCTGCCTTGATGCCTTTGTATGCATGTAT4400.10024697208577496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)