FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378071
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13560.3586627908514538No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG11450.30285316779123495No Hit
CTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT9530.2520690558122681No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG8450.2235029928240992No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC8140.2153034747441618No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC7690.20340094849909143No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7680.20313644791586766No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7500.19837543741783953No Hit
CTCTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT7430.19652393333527302No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC7320.19361442691981134No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT7180.18991141875467835No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6790.1795958960089507No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6500.1719253790954609No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA6130.16213885751618082No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAT5970.15790684818460024No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT5810.15367483885301966No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5630.1489138283549915No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT5330.14097881085827793No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCCTTTTGCGGGTCACCATATT5310.14044980969183038No Hit
ATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA4960.13119228927899784No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG4770.12616677819774594No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4670.12352177236550806No Hit
AATTAATTATGCCTGCTAGATTCTATCCTACCCACACTAAATATTTGCCC4650.12299277119906049No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCCTTTTGCGGGTCACCATA4630.12246377003261291No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTGTGTAACCCATGAA4520.11955426361715128No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG4400.11638025661846585No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4380.11585125545201828No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4380.11585125545201828No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG4360.11532225428557069No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGACACACCCGTG4280.1132062496197804No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4220.11161924612043769No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT4160.11003224262109497No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG4150.1097677420378712No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4110.10870973970497605No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4090.10818073853852848No Hit
TAATCAATTTCAATCTTCTCTTTTCATTTACAGTGAGAGGGCCCACAAAT3840.10156822395793383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)