FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546247
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21470.39304563686390953No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAACCATAT18640.34123757201412547No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18470.3381254267757992No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16230.29711833657667686No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15280.2797269367154419No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14800.2709397031013443No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14080.25775885268019777No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAA12610.23084794973702374No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12600.23066488237006333No Hit
CTCCAAAAGATACCAAAAACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12000.2196808403524413No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11170.20448624889473077No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10370.18984085953790136No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG10110.1850811079969318No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9890.1810536259238037No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9870.18068749118988298No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9510.1740970659793097No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9500.17391399861234935No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTAATGACTTCTTTCCTACTATTCG8850.16201461975992545No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTCTGTGGGTCACCATA8460.1548749924484711No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8220.1504813756414223No Hit
GGATAATATGGTTTAATGCCCTTGTCTAAGGGCAAATATTTGGTAAGGTT8100.14828456723789787No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7940.14535548936653198No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7780.1424264114951661No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7660.1402296030916417No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG7580.13876506415595877No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7540.13803279468811727No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAAC7290.13345611051410808No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7140.13071010000970257No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6930.12686568530353484No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA6590.12064139482688234No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT6540.11972605799208051No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6500.11899378852423903No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6490.11881072115727867No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6310.11551550855199205No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTCTGTGGGTCACCATATT6290.11514937381807132No Hit
CTTTTGGAGTGTGGATCCGCACTCCTCCCGCTTACAGACCACCAAATGCC6150.11258643068062617No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6030.11038962227710176No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC5920.10837588124053771No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG5850.10709440967181513No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC5820.10654520757093403No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5820.10654520757093403No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5820.10654520757093403No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATA5810.10636214020397367No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5670.10379919706652851No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5570.10196852339692483No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5530.10123625392908338No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5480.10032091709428151No Hit
CCCTTAGACAAGGGCATTAAACCATATTATCCTGAACATGCAGTTAATCA5470.10013784972732116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)