FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546247
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG23350.4274623018524587No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16880.3090177154291007No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16130.2952876629070732No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14470.2648984799916521No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAACCATAT14360.26288473895508807No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13900.2544636400749112No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13410.24549333909385315No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13010.23817064441543842No Hit
CTCCAAAAGATACCAAAAACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11890.21766709931587724No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAA11310.20704919203217592No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11090.2030217099590478No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9980.18270123222644702No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9460.17318172914450788No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9160.16768970813569686No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8570.15688873348503515No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8300.1519459145771052No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAAC8180.1497491061735808No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8170.14956603880662045No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8100.14828456723789787No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTCTGTGGGTCACCATA8010.14663696093525455No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTAATGACTTCTTTCCTACTATTCG7570.13858199678899838No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7470.13675132311939472No Hit
GGATAATATGGTTTAATGCCCTTGTCTAAGGGCAAATATTTGGTAAGGTT7470.13675132311939472No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG7330.13418837998194957No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7270.13308997578018736No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7210.13199157157842514No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7180.13144236947754404No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7180.13144236947754404No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA7060.12924556107401963No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7060.12924556107401963No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6490.11881072115727867No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTCTGTGGGTCACCATATT6480.1186276537903183No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6210.11368483488238836No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATA6080.11130495911190359No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5860.10727747703877549No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5840.10691134230485476No Hit
CTTTTGGAGTGTGGATCCGCACTCCTCCCGCTTACAGACCACCAAATGCC5830.1067282749378944No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCAATCAGGCA5760.10544680336917182No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC5590.10233465813084557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)