FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331143
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12470.3765744708479418No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11340.3424502405305261No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7160.2162207867899971No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6750.2038394288872179No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6460.19508188305354485No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT5880.1775667913861987No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5680.17152710460435522No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5220.15763582500611517No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5100.15401201293700909No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5070.15310605991973256No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4820.14555645144242818No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT4800.14495248276424386No Hit
ATATAATACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGTTTGGA4720.14253660805150645No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4680.14132867069513777No Hit
CTCTAGTACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4660.1407247020169534No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4630.1398187489996769No Hit
ACACCAAATACTCTAGTACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG4610.1392147803214925No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA4600.13891279598240033No Hit
GACTACTGTTGTTAGACGACGAGGCAGGACCCCTAGAAGAAGAACTCCCT4450.13438303089601775No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4380.1322691405223725No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT4290.12955128147054293No Hit
GTGTAACCAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4290.12955128147054293No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4260.1286453284532664No Hit
CTCTTATATAATACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGT4230.1277393754359899No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4110.1241155633668838No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT3990.1204917512977777No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT3960.11958579828050117No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3940.11898182960231682No Hit
CCTTTAAGAAACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACCTT3850.11626397055048725No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3840.11596198621139507No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3810.11505603319411856No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3780.11415008017684203No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGACCCCTAGAAGAAGAACTCCCT3750.11324412715956551No Hit
CCTACAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3650.11022428376864377No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA3640.10992229942955159No Hit
ATAACACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGTTTGGAAG3640.10992229942955159No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG3630.10962031509045941No Hit
ACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA3580.10811039339499855No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3560.10750642471681418No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3560.10750642471681418No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3490.10539253434316896No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3480.10509055000407679No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTCTGTGGGTCACCATATT3480.10509055000407679No Hit
CACTCATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT3470.10478856566498462No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3430.10358062830861592No Hit
CTTATATAATACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGTTT3430.10358062830861592No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG3370.10176872227406286No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT3370.10176872227406286No Hit
ACACTAAATACTCTAGTACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG3340.10086276925678635No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3330.10056078491769416No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3330.10056078491769416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)