FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331143
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12400.3744605804742966No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10650.32161332113316604No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA6830.2062553035999553No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT6000.1811906034553048No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5990.18088861911621262No Hit
CTCTAGTACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5380.16246757443159No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5250.1585417780233917No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5140.1552199502933778No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5070.15310605991973256No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5020.1515961382242717No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA5000.15099216954608732No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5000.15099216954608732No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4890.1476703418160734No Hit
ATATAATACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGTTTGGA4880.14736835747698124No Hit
ACACCAAATACTCTAGTACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG4830.14585843578152036No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT4820.14555645144242818No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4750.14344256106878298No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4710.14223462371241427No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4700.1419326393733221No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT4560.13770485862603166No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT4390.1325711248614647No Hit
GACTACTGTTGTTAGACGACGAGGCAGGACCCCTAGAAGAAGAACTCCCT4310.13015525014872728No Hit
GTGTAACCAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4290.12955128147054293No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4280.12924929713145078No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT4110.1241155633668838No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4020.12139770431505421No Hit
CCTACAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3910.11807587658504029No Hit
CTCTTATATAATACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGT3900.11777389224594811No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT3900.11777389224594811No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTCTGTGGGTCACCATATT3830.11566000187230291No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3780.11415008017684203No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG3770.11384809583774985No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT3720.11233817414228897No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG3660.11052626810773593No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA3600.10871436207318289No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3590.10841237773409071No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3540.10690245603862984No Hit
CACTCATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT3530.10660047169953767No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT3510.10599650302135333No Hit
GTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG3440.10388261264770808No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3410.10297665963043157No Hit
ATAACACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGTTTGGAAG3410.10297665963043157No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC3400.1026746752913394No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGACCCCTAGAAGAAGAACTCCCT3370.10176872227406286No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3350.10116475359587852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)