FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361472
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30410.8412823123229461No Hit
TCCATAGAGTGTGTAAATAATGTCTTGTTTGGAAGTAATGATTAACTAAA13150.36379027974504247No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8110.2243603930594901No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT7940.21965740084985835No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC6370.1762238845609065No Hit
GACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGA6210.17179753895184136No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6090.1684777797450425No Hit
ATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG5890.16294484773371104No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5540.15326221671388102No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAAAA5520.1527089235127479No Hit
CTCCAAAAGACACCAAATACTCTAAAACCGTTTCTCTTCCAAAAGTGAGA5360.1482825779036827No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5140.14219635269121814No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAAAA5010.1385999468838527No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4990.13804665368271954No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGT4870.13472689447592068No Hit
GACCTACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGA4770.13196042847025496No Hit
CTCTAAAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4670.12919396246458922No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4650.1286406692634561No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACGTTGACAGACT4590.12698078966005666No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4530.12532091005665721No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4430.1225544440509915No Hit
GTATCCAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGA4420.12227779745042491No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4320.11951133144475921No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4050.11204187322946176No Hit
CACTAATATGGGCCTAAAGTTCAGGCAAATATTGTGGTTTCACATTTCTT4040.11176522662889518No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4000.1106586402266289No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC3960.10955205382436262No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACT3940.10899876062322945No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3710.1026358888101983No Hit
CTAATAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAAAT3690.10208259560906514No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT3660.10125265580736545No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA3620.10014606940509914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)