FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466529
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46761.0022956772247813No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA39460.8458209457504249No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC15560.3335269618823267No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14710.31530730136818935No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT14530.3114490203181367No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14340.30737639032085895No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13660.29280066190954906No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11550.24757303404504327No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT11540.24735868509781814No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC11510.24671563825614268No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10710.22956772247813106No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10340.22163681143080063No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT10200.2186359261696486No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9550.20470324460001416No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA9280.1989158230249352No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9210.1974153803943592No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT9200.19720103144713405No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8700.18648358408587676No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8620.1847687925080756No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8410.18026746461634754No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8360.17919571988022182No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8300.17790962619687092No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC8130.17426569409404344No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7990.1712648088328914No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7590.1626908509438856No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7550.16183345515498498No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA7430.15926126778828326No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7150.15325949726597918No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG7110.15240210147707858No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA6960.1491868672687014No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6880.14747207569090023No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6870.1472577267436751No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6770.14511423727142364No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCTTTGTATGCATGTATT6680.14318509674639732No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6640.14232770095749675No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT6490.13911246674911956No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6350.1361115814879675No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6280.1346111388573915No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6250.13396809201571608No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6250.13396809201571608No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5990.1283950193878623No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5870.1258228320211605No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5840.12517978517948508No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5840.12517978517948508No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5800.12432238939058451No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5790.12410804044335937No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5790.12410804044335937No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5640.12089280623498219No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCATGGTGGTCTCCATGC5580.1196067125516313No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5570.11939236360440615No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5500.11789192097383014No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5370.11510538465990323No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5310.11381929097655237No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT5140.11017535887372489No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG5010.10738882255979801No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG4960.10631707782367227No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4900.1050309841403214No Hit
GAATACATGCATACAAAGGCTTTAAAGCAGGATAACCACATTGTGTAAAA4880.10460228624587109No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT4840.10374489045697052No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4840.10374489045697052No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4750.10181574993194421No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC4740.10160140098471905No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA4680.10031530730136819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)