FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643297
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA51710.8038277809472142No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24420.3796069311686515No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC16390.254781228577158No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16160.2512058971206146No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16090.2501177527642753No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT16080.24996230357051252No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT14550.22617857692481075No Hit
GGATAAGGGTATTAAGCCTTATTATCCAGAACATCTAGTTAATCATTACT13310.20690287689822898No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGTCAATC12910.20068490914771872No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12280.19089160994066506No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11850.1842072946088665No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11820.18374094702757823No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT10880.16912872281387914No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT10850.16866237523259087No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10660.16570884055109847No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCATC10350.16088991554445303No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9950.15467194779394275No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG9930.15436104940641726No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9850.1531174558563152No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACACTTCTT9830.1528065574687897No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9800.1523402098875014No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT9740.15140751472492486No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9660.15016392117482283No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9600.14923122601224628No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9380.14581134374946564No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9210.14316870745549878No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG8950.1391270284176671No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8900.13834978244885332No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8860.13772798567380232No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT8730.13570714615488647No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8650.13446355260478443No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8620.13399720502349613No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8500.13213181469834306No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8450.13135456872952928No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8190.1273128896916976No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8190.1273128896916976No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACAACC8160.12684654211040935No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8050.12513660097901902No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA8030.12482570259149352No Hit
GTCCCGTGCTGGTTGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC8010.12451480420396799No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7850.12202761710376389No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA7830.12171671871623838No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7770.12078402355366184No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG7670.11922953161603428No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7550.1173641412908812No Hit
GTATTAAGCCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7470.11612054774077915No Hit
GTATGGATCGGGAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC7470.11612054774077915No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7450.11580964935325362No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7420.11534330177196536No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7390.1148769541906771No Hit
CTCATAGGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC7360.11441060660938882No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA7360.11441060660938882No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC7350.11425515741562606No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTATCCTTCCGTACG7270.11301156386552401No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7210.11207886870294748No Hit
GGATAATAAGGCTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7050.10959168160274338No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6980.10850353724640407No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6780.10539455337114895No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6590.10244101868965656No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6580.1022855694958938No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC6520.10135287433331727No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6510.10119742513955451No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6500.10104197594579176No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6500.10104197594579176No Hit
CCCTATGAGTGCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6460.10042017917074073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)