FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643297
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA48120.7480215203863845No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC22800.3544241617790849No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC16290.25322673663953044No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT15270.23737091887572925No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14920.2319301970940328No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14710.2286657640250149No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14180.22042695675558877No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT13550.2106336575485351No Hit
GGATAAGGGTATTAAGCCTTATTATCCAGAACATCTAGTTAATCATTACT11990.1863835833215451No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG11340.17627938572696591No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT10960.17037231636398117No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT10930.1699059687826929No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGTCAATC10730.16679698490743777No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10710.16648608651991226No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT10390.16151171231950406No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10370.16120081393197855No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCATC10350.16088991554445303No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9760.1517184131124504No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9680.15047481956234834No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACACTTCTT9680.15047481956234834No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9610.14938667520600904No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9580.14892032762472077No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9150.14223601229292224No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG9110.1416142155178712No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8730.13570714615488647No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8540.1327536114733941No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG8370.13011097517942724No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8180.12715744049793487No Hit
GTCCCGTGCTGGTTGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC8110.12606929614159557No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8050.12513660097901902No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7850.12202761710376389No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7840.12187216791000113No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT7820.12156126952247563No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7800.1212503711349501No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTATCCTTCCGTACG7790.12109492194118734No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7630.11860773484098325No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7620.11845228564722049No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7560.11751959048464394No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7480.1162759969345419No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACAACC7480.1162759969345419No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7470.11612054774077915No Hit
GTATGGATCGGGAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC7400.11503240338443985No Hit
GTATTAAGCCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7300.11347791144681227No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA7290.11332246225304952No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7240.11254521628423574No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC7220.11223431789671022No Hit
GGATAATAAGGCTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7190.11176797031542196No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7190.11176797031542196No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT7100.11036892757155714No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7060.10974713079650612No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA7020.10912533402145509No Hit
CTCTACTACTACTTCTCTTCCAAAAGTGAGACAAGAAGTGTGAAACCACA6850.10648269772748824No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6810.1058609009524372No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6780.10539455337114895No Hit
GTATCGGGAAGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG6770.10523910417738619No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6660.10352916304599585No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA6600.10259646788341932No Hit
CCCTATGAGTGCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6600.10259646788341932No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6530.10150832352708002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)