FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363982
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT16550.4546928144798369No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12860.35331417487677963No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA12040.33078558829832244No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9980.2741893829914666No Hit
CTACATCCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT9770.2684198669164959No Hit
ACTCACAACTGTGCCAGCAACTCCTCCTCCTGCCTCCACCAATCGGCAAT8940.2456165414773258No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7550.20742783983823376No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7510.20632888439538216No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAA7330.20138358490255012No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC7130.1958888076882923No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7030.19314141908116336No Hit
ACATCCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTT7010.19259194135973756No Hit
CTACTTGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT6650.18270134237407346No Hit
GTATAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC6620.18187712579193477No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT6480.17803078174195427No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6400.17583287085625113No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA6250.17171178794555775No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT6220.17088757136341906No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5890.1618211889598936No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5470.15028215680995213No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT5280.1450621184564072No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC5270.1447873795956943No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5250.1442379018742685No Hit
GAGGATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCT5230.14368842415284272No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA5060.13901786352072354No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4940.13572099719216885No Hit
CTTCAAGATCCCAGAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAG4720.1296767422564852No Hit
GAGCAAGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCT4610.1266546147886434No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4510.12390722618151448No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4390.12061035985295977No Hit
ACTACATCCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4280.11758823238511794No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC4260.11703875466369217No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTATAAGGCC4180.11484084377798903No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4140.11374188833513746No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4120.11319241061371167No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT4110.11291767175299877No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG4110.11291767175299877No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4100.11264293289228587No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4020.11044502200658274No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4020.11044502200658274No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG3930.10797237226016672No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3930.10797237226016672No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3910.10742289453874092No Hit
TTATTATCCAGAGGATGTAGTTAATCATTACTTCCAGACGAGACATTATT3900.10714815567802803No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3890.10687341681731514No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA3870.10632393909588936No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC3840.10549972251375067No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3840.10549972251375067No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT3810.10467550593161201No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTACTGACTTTTTTCCTTCTATGCG3740.10275233390662177No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3740.10275233390662177No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3690.10137863960305729No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTT3680.1011039007423444No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA3680.1011039007423444No Hit
AATCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3650.10027968416020572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)