FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363982
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT17170.47172662384403624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12840.35276469715535386No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10870.298641141594914No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC8010.2200658274310268No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7990.21951634970960102No Hit
CTACATCCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT7810.21457105021676898No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7750.2129226170524916No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC7690.21127418388821426No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAA7600.20880153414179822No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7130.1958888076882923No Hit
ACTCACAACTGTGCCAGCAACTCCTCCTCCTGCCTCCACCAATCGGCAAT6500.17858025946338005No Hit
GTATAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC6470.1777560428812414No Hit
CTACTTGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT6180.1697886159205675No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT5990.16456857756702253No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT5590.15357902313850685No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5590.15357902313850685No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5580.15330428427779397No Hit
ACATCCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTT5430.1491832013671006No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5390.148084245924249No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA5330.14643581275997164No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5280.1450621184564072No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5220.14341368529212983No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5120.1406662966850009No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATCCTCTGGAT4900.13462204174931727No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT4780.13132517542076258No Hit
GAGGATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCT4760.13077569769933678No Hit
GAGCAAGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCT4730.1299514811171981No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC4520.12418196504222737No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4110.11291767175299877No Hit
CTTCAAGATCCCAGAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAG4080.1120934551708601No Hit
AATCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3910.10742289453874092No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG3810.10467550593161201No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3760.10330181162804754No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA3750.10302707276733465No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3750.10302707276733465No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3730.10247759504590888No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3700.10165337846377018No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT3690.10137863960305729No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3690.10137863960305729No Hit
TTATTATCCAGAGGATGTAGTTAATCATTACTTCCAGACGAGACATTATT3680.1011039007423444No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3670.10082916188163152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)