FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552705
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24600.44508372459087575No Hit
ATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG22740.41143105273156566No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT15900.2876760658941026No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14900.2695832315611402No Hit
ACTCTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG14270.2581847459313739No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13990.2531187523181444No Hit
CTGTCTGATGTGATCTTGTGGTAAGGACCCATAACATCCAATGACATAGC13750.24877647207823345No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA13350.24153933834504845No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAG12960.23448313295519307No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12920.2337594195818746No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC12540.22688414253534886No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12000.21711401199554914No Hit
ATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAG11840.2142191585022752No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA11780.21313358844229743No Hit
CTTTTACACAATGTGGTTATCCCGCTTTAATGCCCTTGTATGCATGTATT11730.2122289467256493No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11290.20426809961914583No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCAAACAAA10690.19341239901936838No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGT10300.18635619362951303No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10190.18436598185288716No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9710.1756814213730652No Hit
TCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAATACTAATATGGGCC9680.17513863634307633No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT9560.17296749622312085No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9260.1675396459232321No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT9120.16500664911661736No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8980.16247365231000263No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8970.162292723966673No Hit
ACTCTGGAGTTACTCTCGTTTTTGTTTTCGGAAACTTCCTGTTAACAGGC8650.156503016980125No Hit
CTCTTATATAAGATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGT8440.1527035217702029No Hit
TACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8290.14998959662025854No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8140.1472756714703142No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8070.1460091730670068No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7920.14329524791706244No Hit
GGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC7880.14257153454374394No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7810.14130503614043657No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7790.14094317945377732No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG7710.13949575270714032No Hit
GAATACATGCATACAAGGGCATTAAAGCGGGATAACCACATTGTGTAAAA7710.13949575270714032No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7700.1393148243638107No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7640.13822925430383295No Hit
CTTATATAAGATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTT7520.13605811418387748No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7390.13370604572059236No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG7380.13352511737726275No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7350.13298233234727386No Hit
CCAGTATCCAGGGACCTAGTAGTCAGTTATGTTAATACTAATATGGGCCT7270.13153490560063688No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7210.13044933554065913No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACA7210.13044933554065913No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7160.129544693824011No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC7140.12918283713735176No Hit
CACTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACGTAGCGCC7020.12701169701739626No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6960.1259261269574185No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6960.1259261269574185No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6900.12484055689744077No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6840.12375498683746301No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6800.12303127346414452No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6780.12266941677748527No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6700.12122199003084827No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6680.12086013334418903No Hit
CATGCATACAAGGGCATTAAAGCGGGATAACCACATTGTGTAAAAGGGGC6640.12013641997087054No Hit
GGATGTTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA6590.11923177825422242No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC6490.11742249482092618No Hit
CATGTATTCAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCT6480.11724156647759654No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA6460.1168797097909373No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6360.11507042635764105No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6310.11416578464099293No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6300.11398485629766332No Hit
CATCATACAGAAAATCAAAGAATGTTTTCGAAAACTTCCTGTTAACAGGC6260.1132611429243448No Hit
ATATAAGATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGA6170.11163278783437819No Hit
CTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA6160.11145185949104858No Hit
TGTTTACACAGAAAAGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTT6060.10964257605775234No Hit
CTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTTACTTTC6060.10964257605775234No Hit
TCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTG6020.10891886268443382No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5960.10783329262445608No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5920.1071095792511376No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5770.10439565410119322No Hit
GTACCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5740.10385286907120435No Hit
TACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5720.1034910123845451No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5720.1034910123845451No Hit
CTCCGAAAGACACCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGA5720.1034910123845451No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5700.10312915569788586No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5640.10204358563790811No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAACACC5580.10095801557793037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)