FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552705
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA25140.4548538551306755No Hit
ATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG18170.3287467998299274No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT17370.3142725323635574No Hit
ACTCTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG16590.30016012158384675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13990.2531187523181444No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13720.24823368704824456No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13590.24588161858495944No Hit
CTGTCTGATGTGATCTTGTGGTAAGGACCCATAACATCCAATGACATAGC13580.24570069024162983No Hit
CTTTTACACAATGTGGTTATCCCGCTTTAATGCCCTTGTATGCATGTATT12890.2332166345518857No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12860.23267384952189685No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA12560.22724599922200814No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA12030.21765679702553803No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10690.19341239901936838No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10570.1912412588994129No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGT10540.190698473869424No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAG10000.1809283433296243No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9950.18002370161297618No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCAAACAAA9790.1771288481197022No Hit
ATAATATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAG9790.1771288481197022No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9490.17170099781981346No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9480.17152006947648385No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9250.16735871757990248No Hit
CTCTTATATAAGATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGT9150.16554943414660625No Hit
ACTCTGGAGTTACTCTCGTTTTTGTTTTCGGAAACTTCCTGTTAACAGGC9140.1653685058032766No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8840.1599406555033879No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8760.1584932287567509No Hit
TCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAATACTAATATGGGCC8670.15686487366678428No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8590.15541744692014728No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8560.1548746618901584No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8520.15415094851683991No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8330.15071330999357704No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT8070.1460091730670068No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8070.1460091730670068No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7930.14347617626039205No Hit
GGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC7760.14040039442378846No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7680.13895296767715148No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC7560.13678182755719598No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACA7530.13623904252720712No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7280.1317158339439665No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7270.13153490560063688No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7260.13135397725730724No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7250.1311730489139776No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7240.130992120570648No Hit
TACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7190.13008747885399988No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC6890.12465962855411114No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6890.12465962855411114No Hit
GAATACATGCATACAAGGGCATTAAAGCGGGATAACCACATTGTGTAAAA6840.12375498683746301No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6620.1197745632842113No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6580.11905084991089279No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6540.11832713653757429No Hit
CTCCGAAAGACACCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGA6500.1176034231642558No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT6490.11742249482092618No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6400.11579413973095956No Hit
CTCTCGTCCCCTCCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT6370.11525135470097068No Hit
CTTATATAAGATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTT6370.11525135470097068No Hit
GTACCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG6330.1145276413276522No Hit
CACTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACGTAGCGCC6280.11362299961100407No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6260.1132611429243448No Hit
CATCATACAGAAAATCAAAGAATGTTTTCGAAAACTTCCTGTTAACAGGC6200.11217557286436707No Hit
CTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA6140.11109000280438931No Hit
CTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTTACTTTC6120.11072814611773006No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6050.1094616477144227No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6020.10891886268443382No Hit
CCAGTATCCAGGGACCTAGTAGTCAGTTATGTTAATACTAATATGGGCCT5990.10837607765444496No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5990.10837607765444496No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5990.10837607765444496No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5940.10747143593779684No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5920.1071095792511376No Hit
GGATGTTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA5910.10692865090780797No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5850.1058430808478302No Hit
CATGCATACAAGGGCATTAAAGCGGGATAACCACATTGTGTAAAAGGGGC5810.10511936747451171No Hit
ATATAAGATACCCGCCTTCCATAGTGTGTGTAAATAATGTCTGGTTTGGA5630.1018626572945785No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAACACC5620.10168172895124886No Hit
TGTTTACACAGAAAAGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGCTT5550.10041523054794149No Hit
ATGTGGTTATCCCGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC5540.10023430220461185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)