FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418806
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA41140.9823163947030367No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT19020.4541482213721866No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17430.41618314923854954No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15860.37869562518206523No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12040.28748394244590575No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11270.2690983414755281No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11070.2643228607040014No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT10740.25644331743098236No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10600.2531004808909137No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10550.25190661069803205No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10530.2514290626208794No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT10240.24450461550216568No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10090.24092300492352067No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9850.23519242799768866No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9780.23352100972765436No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9240.2206272116445323No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT9240.2206272116445323No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9150.2184782452973453No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8830.21083747606290265No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8710.20797218759998665No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8520.20343548086703628No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8480.20248038471273097No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8380.20009264432696763No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT8170.1950783895168646No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7980.19054168278391428No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7980.19054168278391428No Hit
ACAACAGAGTTATCCGTCCCGAGAATGTTTGCCCCAGACCTGCTGCGAGC7790.18600497605096394No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7740.18481110585808228No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7730.18457233181950594No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7670.18313968758804794No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7620.18194581739516627No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCTACCGTG7540.1800356250865556No Hit
CAGTTAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTAT7360.17573769239218157No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7250.1731111779678419No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7180.1714397596978076No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7170.17120098565923125No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7080.16905201931204425No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT6950.16594795681055188No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6940.16570918277197558No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6880.16427653854051757No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6780.16188879815475424No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6700.15997860584614354No Hit
GTTAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC6490.15496435103604056No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6330.15114396641881922No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6320.1509051923802429No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5920.14135423083718954No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5870.14016036064430787No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5690.13586242794993386No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC5670.1353848798727812No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5660.13514610583420486No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5580.1332359135255942No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5560.13275836544844152No Hit
CTCACAGTTAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5550.1325195914098652No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5450.13013185102410185No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5440.12989307698552552No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5430.12965430294694918No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC5400.12893798083122018No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5360.12798288467691485No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5290.1263114664068805No Hit
CTCTATTGTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGTT5290.1263114664068805No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5280.12607269236830418No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5270.12583391832972785No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5240.12511759621399884No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5210.12440127409826984No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT5190.12392372602111719No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5170.12344617794396451No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG5160.12320740390538817No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5120.12225230775108284No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5060.12081966351962484No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA4990.11914824524959051No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4970.11867069717243783No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4960.1184319231338615No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4910.11723805294097983No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4870.1162829567866745No Hit
ACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAA4830.11532786063236916No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGACTGCAATTGATTATG4830.11532786063236916No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4800.11461153851664016No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC4770.11389521640091116No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG4760.11365644236233483No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4750.11341766832375849No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4680.11174625005372416No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4640.11079115389941883No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4590.10959728370653717No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA4590.10959728370653717No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT4580.10935850966796082No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA4540.1084034135136555No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4520.10792586543650282No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4460.10649322120504481No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC4430.10577689908931583No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4390.10482180293501049No Hit
CACTCACAGTTAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGG4390.10482180293501049No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4380.10458302889643416No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4340.10362793274212882No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4340.10362793274212882No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4310.10291161062639981No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4240.10124019235636549No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4230.10100141831778915No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4230.10100141831778915No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4220.1007626442792128No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4220.1007626442792128No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA4210.10052387024063647No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA4210.10052387024063647No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4210.10052387024063647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)