FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493871
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT18480.37418678156846624No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA17590.35616588137388105No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17350.35130631278208274No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT15530.31445458429427925No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14690.29744609422298535No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA13410.27152839506672793No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC13260.268491164696854No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT12850.26018940168586535No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA12800.25917699156257407No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG11210.22698234964191052No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC10960.22192029902545402No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10620.21503591018707316No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT10290.20835400337335053No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10190.20632918312676793No Hit
GTCTAGAAGGTCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA9820.19883734821441226No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT9260.18749835483354965No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT9200.1862834626856001No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9160.18547353458696705No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9000.18223382219243486No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8870.17960155587187748No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGA8790.1779816996746114No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA8780.1777792176499531No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8580.1737295771567879No Hit
CTTCTAGACACCGCCTCAGCGCTGTATCGGGAAGCCTTAGAGTCTCCTGA7590.15368385671562007No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7500.15186151849369572No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC7460.1510515903950627No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC7410.15003918027177138No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA7330.14841932407450528No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7120.1441672015566818No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7120.1441672015566818No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7090.14355975548270702No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC7040.14254734535941574No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA6990.14153493523612443No Hit
CCCTTATCCAATGGCAAATATTTTGTAACATTTGGATAAAACCTAGCAGG6860.13890266891556702No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6850.13870018689090877No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA6820.13809274081693398No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6800.13768777676761745No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6760.13687784866898442No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6750.13667536664432614No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6700.13566295652103483No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT6530.1322207621018444No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6520.13201828007718613No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6470.13100586995389485No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC6430.1301959418552618No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACT6410.12979097780594526No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6390.12938601375662875No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTAAGA6300.1275636755347044No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6270.12695622946072962No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6220.1259438193374383No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC6200.12553885528812178No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTTGTAACATTTGGATAAAAC6190.12533637326346353No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6180.12513389123880528No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6070.1229065889675644No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6070.1229065889675644No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6020.12189417884427312No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6000.12148921479495657No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG5890.11926191252371571No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5890.11926191252371571No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG5860.11865446644974093No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATGTGGGCC5840.11824950240042441No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5770.11683212822781658No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5770.11683212822781658No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5760.11662964620315831No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5750.11642716417850005No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGATCCTTAT5690.1152122720305505No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT5670.11480730798123395No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5630.11399737988260093No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG5610.1135924158332844No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT5570.11278248773465135No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5560.1125800057099931No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5550.11237752368533484No Hit
GCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG5520.11177007761136006No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5510.11156759558670179No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5490.11116263153738527No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5480.11096014951272701No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5470.11075766748806874No Hit
CCTTCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5430.1099477393894357No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5420.10974525736477743No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5390.10913781129080265No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5370.10873284724148614No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5360.10853036521682788No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT5300.10731547306887831No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5300.10731547306887831No Hit
ACTACACACCGTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGT5240.10610058092092875No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG5220.10569561687161222No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5200.1052906528222957No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5190.10508817079763744No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5180.10488568877297917No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG5170.10468320674832093No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5160.10448072472366265No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC5160.10448072472366265No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5120.10367079662502961No Hit
ATCCAGTATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATGTGGGC5110.10346831460037134No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT5090.10306335055105484No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5070.1026583865017383No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT5060.10245590447708006No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5040.10205094042776353No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT4980.10083604827981396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)