FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573716
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA47530.8284586799043429No Hit
ATCACAAAATGTGAACAATTTGTAGGCCCACTTACAGTCAATGAGAAAAG24470.42651764984765983No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC22770.39688626428407087No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20750.36167720614380633No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT17680.30816640986132515No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT17130.298579785120164No Hit
TCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA15760.2747003744012717No Hit
CCTAACATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGT15720.2740031653291873No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15520.27051711996876504No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15430.268948399556575No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT15070.262673517907815No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT14630.2550042181148861No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14360.2502980568783161No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC13750.23966561852902832No Hit
CCTAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTC13340.23251922554016272No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC13280.23147341193203605No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT13050.2274644597675505No Hit
ATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGTCAATGAGAAAAG13010.22676725069546605No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT12750.22223539172691714No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12380.21578620781013605No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA12170.21212586018169272No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA12040.20985993069741823No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11590.2020163286364682No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA11500.20044760822427823No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC11440.19940179461615154No Hit
GGATAAGGGTATTAAACCGTATTATCCAGAACATCTAGTTAATCATTACT11120.19382412203947597No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT11090.19330121523541266No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10960.1910352857511382No Hit
CTAACATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA10910.19016377441103263No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT10900.1899894721430115No Hit
CATTTAAACCCGAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT10850.18911796080290597No Hit
GGATAATACGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT10800.18824644946280042No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT10280.1791827315257026No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA10260.1788341269896604No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT10220.17813691791757594No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA10100.1760452907013226No Hit
GTATTAAACCGTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10070.17552238389725927No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9570.1668072704962037No Hit
CTTACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT9560.16663296822818258No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9500.1655871546200559No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT9430.16436703874390812No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG9120.1589636684352537No Hit
AATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTTGTCT8990.15669773895097922No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8840.15408320493066258No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8810.15356029812659922No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCGTAT8650.15077146183826146No Hit
GTTCTGGATAATACGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8520.148505532353987No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG8500.14815692781794476No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT8290.14449658018950143No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC8090.1410105348290792No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA8040.14013902348897364No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC7890.13752448946865697No Hit
CACTTACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGG7820.1363043735925092No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7820.1363043735925092No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7800.13595576905646697No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC7660.1335155373041714No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA7440.12968088740770695No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7400.12898367833562252No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7220.1258462375112425No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7170.12497472617113695No Hit
GTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGAACA7130.12427751709905249No Hit
GTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7120.12410321483103139No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7060.1230574012229047No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6900.12026856493456692No Hit
AGTTCAGACAACTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGA6780.11817693771831358No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6710.11695682184216581No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA6710.11695682184216581No Hit
CCTATAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT6700.1167825195741447No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6630.11556240369799693No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG6630.11556240369799693No Hit
AATGCGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTTGTCT6550.11416798555382802No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6470.11277356740965915No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGT6420.11190205606955358No Hit
TTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCT6400.11155345153351136No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC6390.11137914926549024No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6380.11120484699746913No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6350.11068194019340581No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6340.11050763792538468No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA6280.10946182431725801No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6270.1092875220492369No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6190.10789310390506802No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6150.10719589483298357No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6010.10475566308068801No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6000.10458136081266689No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5890.10266403586443468No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5870.10231543132839245No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5810.10126961772026577No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5800.10109531545224466No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5780.10074671091620244No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC5760.10039810638016022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)