FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573716
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA47810.833339143408934No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC22480.3918314985114586No Hit
ATCACAAAATGTGAACAATTTGTAGGCCCACTTACAGTCAATGAGAAAAG21160.36882359913267193No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20650.35993418346359524No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA17260.30084571460443843No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC16860.29387362388359395No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT16720.29143339213129843No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT15960.27818641976169395No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15510.2703428177007439No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT15490.2699942131647017No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT15480.26981991089668056No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT15130.2637193315159417No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT14800.25796735667124504No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14630.2550042181148861No Hit
CCTAACATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGT13320.2321706210041205No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA13180.22973038925182496No Hit
GGATAATACGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT12420.21648341688222045No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11960.20846551255324935No Hit
GGATAAGGGTATTAAACCGTATTATCCAGAACATCTAGTTAATCATTACT11830.20619958306897487No Hit
ATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGTCAATGAGAAAAG11650.20306214224459487No Hit
CCTAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTC11630.20271353770855266No Hit
GTATTAAACCGTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC11490.2002733059562571No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT11370.19818167874000375No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11250.19609005152375045No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11000.19173249482322263No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA10980.19138389028718042No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA10610.1849347063703993No Hit
CATTTAAACCCGAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT10520.1833659859582093No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA10460.18232017235008263No Hit
TCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA10450.1821458700820615No Hit
CTAACATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA10290.17935703379372372No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT10100.1760452907013226No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT10090.1758709884333015No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9910.17273354760892148No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG9820.17116482719673148No Hit
GTTCTGGATAATACGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA9380.16349552740380258No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9190.16018378431140146No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCGTAT9090.15844076163119036No Hit
GTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT9070.15809215709514812No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8910.15530332080681034No Hit
AATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTTGTCT8730.15216587998243034No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT8700.151642973178367No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC8580.14955134596211367No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8570.14937704369409255No Hit
CTTACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT8420.14676250967377588No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT8400.14641390513773367No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8370.14589099833367034No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8140.14188204616918476No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG8080.1408362325610581No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA8010.1396161166849103No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7780.13560716452042473No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7770.13543286225240364No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA7700.13421274637625585No Hit
GTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGAACA7640.1331669327681292No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7560.1317725146239603No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA7450.12985518967572807No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA7380.12863507379958028No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7240.12619484204728473No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC7210.1256719352432214No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7150.12462612163509472No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7060.1230574012229047No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6940.12096577400665137No Hit
CACTTACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGG6820.11887414679039804No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6650.11591100823403913No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6450.11242496287361692No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6440.1122506606055958No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC6430.11207635833757469No Hit
TTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCT6420.11190205606955358No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6380.11120484699746913No Hit
AGTTCAGACAACTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGA6270.1092875220492369No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6240.10876461524517357No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6170.1075444993690258No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6160.10737019710100468No Hit
GGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6030.10510426761673024No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA6020.10492996534870912No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5960.10388415174058245No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTCGGGTTTAAATGTATAC5850.10196682679235022No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5840.10179252452432912No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5810.10126961772026577No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5800.10109531545224466No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5780.10074671091620244No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC5770.10057240864818134No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5750.10022380411213912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)