FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437146
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46121.0550250945908233No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA28710.6567599840785459No Hit
GTGTAAAAGGAGCAGCAAAACCTAAAAGACCCACAATACGTTGACAGACT13270.30355990904640556No Hit
CATTTAAACCCTAACAAAACCAAAAGATGGGGTTACTCTTTAAATTTCAT10500.24019435154387778No Hit
CTACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAAT10300.2356192210382801No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10280.23516170798772038No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10080.2305865774821227No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9920.22692647307764455No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9560.21869123816756872No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG8840.20222076834741712No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT8770.2006194726704579No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8680.19856066394293898No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7840.17934511581942875No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA7810.1786588462435891No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTAT7460.17065236785879317No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG7400.16927982870711386No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC7360.16836480260599432No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7250.16584848082791562No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTACTGTGGTTTCACATTTCTT7050.16127335032231793No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6860.15692697634200015No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6840.1564694632914404No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC6820.15601195024088063No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6810.15578319371560073No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6800.15555443719032086No Hit
ACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAATAT6700.15326687193752203No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6660.1523518458364025No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT6430.14709044575496516No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCTAAAAGACCCACAATACG6390.14617541965384562No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6040.1381689412690497No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5810.1329075411876124No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5760.13176375856121295No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5530.12650235847977565No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5510.12604484542921587No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5420.1239860367016969No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5350.12238474102473772No Hit
CAGTAAATGAGAAACGAAGACTGCAATTGATTATGCCTGCTAGGTTTTAT5330.12192722797417796No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5320.12169847144889807No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT5310.1214697149236182No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG5140.11758085399386019No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTATACCTGTC5130.11735209746858029No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5110.11689458441802052No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5100.11666582789274065No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4940.11300572348826252No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGCAG4930.11277696696298262No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATTAGGGTTAAAGA4920.11254821043770273No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4890.11186194086186309No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4850.11094691476074355No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4830.11048940171018379No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCTAAAAGACCCACAAT4760.1088881060332246No Hit
TGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT4760.1088881060332246No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC4730.10820183645738496No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4700.1075155668815453No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4640.10614302772986599No Hit
CCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC4630.10591427120458612No Hit
CTCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAG4610.10545675815402634No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG4610.10545675815402634No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4590.10499924510346659No Hit
ACCTAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGTCTGT4540.10385546247706715No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4510.1031691929012275No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA4440.10156789722426832No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTCTAATTTTCTGT4430.10133914069898844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)