FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437146
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46021.0527375293380243No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA25290.5785252524328256No Hit
GTGTAAAAGGAGCAGCAAAACCTAAAAGACCCACAATACGTTGACAGACT12020.2749653433864201No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10170.23264538620964165No Hit
CATTTAAACCCTAACAAAACCAAAAGATGGGGTTACTCTTTAAATTTCAT9420.21548864681365035No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG9390.2148023772378107No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9370.21434486418725096No Hit
CTACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAAT9100.2081684380046941No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8830.20199201182213722No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC8800.20130574224629755No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8780.20084822919573783No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8770.2006194726704579No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8720.19947569004405852No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT8580.19627309869014015No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8190.18735159420422468No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG8000.1830052202239069No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTAT7870.1800313853952684No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7690.1759137679402305No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT7420.16973734175767363No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA7320.1674497765048748No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7210.1649334547267961No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6820.15601195024088063No Hit
ACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAATAT6290.1438878544010468No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6290.1438878544010468No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6060.1386264543196095No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTACTGTGGTTTCACATTTCTT6020.13771142821848995No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5920.13542386296569112No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5720.13084873246009343No Hit
ACCCATCTCGGGGCCGCTTGGGGATCTTTCGTCCCCTTCTCCGTCTGCCG5530.12650235847977565No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5500.12581608890393597No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC5480.12535857585337623No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5480.12535857585337623No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCTAAAAGACCCACAAT5460.12490106280281645No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5370.1228422540752975No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5370.1228422540752975No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCTAAAAGACCCACAATACG5330.12192722797417796No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATTAGGGTTAAAGA5330.12192722797417796No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5100.11666582789274065No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5020.11483577569050157No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4960.11346323653882227No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4920.11254821043770273No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG4910.11231945391242285No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4870.11140442781130333No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4780.10934561908378436No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGCAG4740.10843059298266482No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTATACCTGTC4730.10820183645738496No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4720.10797307993210506No Hit
TGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT4620.10568551467930623No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTCTAATTTTCTGTATGATGTGAT4570.1045417320529068No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC4550.10408421900234704No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4510.1031691929012275No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4440.10156789722426832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)