FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492418
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTACATGTTCTGGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATA48010.9749846674979388No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA43630.8860358475928988No Hit
CACCCAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT28510.5789796473727605No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG26480.5377545093802419No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGT21770.4421040660576989No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG16290.33081650142764885No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16150.32797338846264756No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT16060.3261456729851468No Hit
CCCCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15730.3194440495676438No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAAT15070.3060408027326377No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT14530.2950745098676328No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC13620.27659427559512445No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA13250.26908033418762106No Hit
ACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACC13050.26501874423761923No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT12430.25242781539261355No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA11440.23232294514010454No Hit
GACTACAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATAT11050.22440284473760097No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCC10840.22013817529009908No Hit
CAATTACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG10550.2142488698625964No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA10540.21404579036509633No Hit
GCAATGACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAA10430.2118119158925953No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10390.21099959790259495No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10370.21059343890759474No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA10220.2075472464450934No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT10150.20612568996259276No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT9920.20145486152009065No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9690.19678403307758857No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA9530.1935347611175871No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG9380.19048856865508573No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA9360.19008240966008552No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9290.1886608531775849No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG9040.18358386574008262No Hit
ACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTAT9020.18317770674508244No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8660.17586684483507914No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8490.17241449337757758No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTC8390.17038369840257667No Hit
GTCTGGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGT7940.16124512101507255No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7890.16022972352757212No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC7880.160026644030072No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA7530.15291886161756882No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7500.15230962312506854No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7400.15027882815006763No Hit
GGTCCTGCATGATCCCGTGCTGGTCGTTGAAGATCCTGGAATTAGAGGAC7270.14763879468256644No Hit
CTACATGTTCTGGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATAT7100.14418644322506488No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7020.14256180724506415No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAT6910.14032793277256314No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC6670.13545402483256097No Hit
GCAAACCCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGG6630.13464170684256058No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT6620.13443862734506049No Hit
GTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACA6590.1338293888525602No Hit
GCTCCGGAGACTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGA6570.13342322985756003No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6540.13281399136505978No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT6490.13179859387755932No Hit
CCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCT6480.13159551438005923No Hit
GGATACTGGATCTTCCAAATTTCCACCCACCCAGGTAGCTAGAGTCATTA6470.1313924348825591No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6460.13118935538505905No Hit
CCTCACCATACTGCACTAAGGCAAGCAATTCTGTGCTGGGGGGACCTAAT6390.1297677989025584No Hit
GGGTAAAGGTTCAGGTATTGTTTACAAAGAAAGGCCTTGTAAGTTGGCGA6300.12794008342505758No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG6290.1277370039275575No Hit
CACTAATATGGGCCTAAAGTTCCGACAACTATTGTGGTTTCACATTTCTT6250.1269246859375571No Hit
ACCGAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA6180.12550312945505648No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6130.12448773196755603No Hit
CTAGATGTTCTGGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATAT6060.12306617548505538No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG6050.12286309598755528No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTC6050.12286309598755528No Hit
ATCTCAATCTCGGGAATCTTAATGTTAGTATTCCTTGGACTCATAAGGTG6000.12184769850005484No Hit
GCTGTGGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAAC5910.12001998302255401No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAA5910.12001998302255401No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA5820.11819226754505319No Hit
GAATACATGCATATAATGGCATTAAAGCAGGGTAACCACATTGTGTGAAA5800.11778610855005299No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5790.11758302905255291No Hit
GGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5760.11697379056005264No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT5750.11677071106255255No Hit
ATCAACTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTACAAATTG5690.115552234077552No Hit
GTTCAGCGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGATA5650.11473991608755163No Hit
ACTAGATGTTCTGGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATA5640.11453683659005155No Hit
AAGCTGTGGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATA5580.113318359605051No Hit
GGTCCTTGCCACAAGATCACATCATACAAAAAATCAAAGAATGTTTTCGA5530.11230296211755053No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5500.11169372362505026No Hit
ATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTCACGGTGGTCTCCATGC5500.11169372362505026No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5490.11149064412755018No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA5450.11067832613754981No Hit
GAAACGATGTATATTTGCGGGAAAGAACAACAGAGTTATCCGTCCCGAGA5430.11027216714254963No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAGTCTTTGA5400.10966292865004934No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5370.10905369015754907No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACA5360.10885061066004899No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG5350.10864753116254888No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5320.10803829267004862No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5300.10763213367504844No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAA5290.10742905417754835No Hit
GCATGATCCCGTGCTGGTCGTTGAAGATCCTGGAATTAGAGGACAAACGG5280.10722597468004824No Hit
GTCTATTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTTTGTATGATG5250.10661673618754797No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5220.10600749769504769No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTTGGACGGAAATTGCACC5190.10539825920254745No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5100.10357054372504661No Hit
CTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTAT5070.10296130523254633No Hit
GAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTCGTTGAAGAT5040.10235206674004606No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5010.10174282824754578No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT4990.1013366692525456No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4990.1013366692525456No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA4950.10052435126254523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)