FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492418
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTACATGTTCTGGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATA40320.8188165339203685No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA37830.7682497390428457No Hit
CACCCAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT27840.5653733210402545No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG27050.5493300407377472No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGT24360.49470165591022264No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG16450.3340657733876503No Hit
CCCCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15710.31903789057264353No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAAT15680.3184286520801433No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT15310.3109147106726399No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA15230.3092900746926392No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15140.30746235921513837No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14690.29832378182763425No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13010.26420642624761886No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT12940.2627848697651182No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12480.25344321288011407No Hit
GACTACAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATAT11730.23821225056760723No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11280.2290736731801031No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA11220.22785519619510256No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11150.2264336397126019No Hit
ACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACC10840.22013817529009908No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT10780.21891969830509853No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA10490.21303039287759584No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT10170.20653184895759294No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA10150.20612568996259276No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCC9880.2006425435300903No Hit
GTCTGGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGT9250.18784853518758454No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA9180.1864269787050839No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9120.18520850172008335No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8940.1815530707650817No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG8900.18074075277508134No Hit
GCAATGACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAA8770.17810071930758015No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG8570.17403912935757831No Hit
CAATTACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG8320.16896214192007603No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC8260.16774366493507548No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTC8070.16388515448257374No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA8030.1630728364925734No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG7820.15880816704507145No Hit
CTACATGTTCTGGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATAT7550.153325020612569No Hit
GTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACA7520.15271578212006873No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7400.15027882815006763No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA7390.15007574865256754No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7300.1482480331750667No Hit
ACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTAT7270.14763879468256644No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7210.1464203176975659No Hit
GCAAACCCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGG7110.14438952272256497No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6920.14053101227006323No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6820.13850021729506232No Hit
GGATACTGGATCTTCCAAATTTCCACCCACCCAGGTAGCTAGAGTCATTA6810.13829713779756223No Hit
GGTCCTGCATGATCCCGTGCTGGTCGTTGAAGATCCTGGAATTAGAGGAC6640.1348447863400607No Hit
GTCTATTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTTTGTATGATG6290.1277370039275575No Hit
GCTCCGGAGACTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGA6220.12631544744505685No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6180.12550312945505648No Hit
GGGTAAAGGTTCAGGTATTGTTTACAAAGAAAGGCCTTGTAAGTTGGCGA6130.12448773196755603No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAT6100.12387849347505575No Hit
GGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6070.12326925498255548No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT6060.12306617548505538No Hit
GGTCCTTGCCACAAGATCACATCATACAAAAAATCAAAGAATGTTTTCGA6050.12286309598755528No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5980.12144153950505465No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5960.12103538051005445No Hit
CTAGATGTTCTGGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATAT5850.11880150603755346No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA5820.11819226754505319No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT5820.11819226754505319No Hit
AAGCTGTGGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATA5800.11778610855005299No Hit
ATCAACTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTACAAATTG5760.11697379056005264No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC5760.11697379056005264No Hit
CTCCGAAAGACACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGA5730.11636455206755236No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5690.115552234077552No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACA5580.113318359605051No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5570.1131152801075509No Hit
CACTAATATGGGCCTAAAGTTCCGACAACTATTGTGGTTTCACATTTCTT5560.1129122006100508No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5540.11250604161505062No Hit
GCTGTGGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAAC5530.11230296211755053No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA5520.11209988262005045No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5410.10986600814754943No Hit
CCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCT5410.10986600814754943No Hit
ACCGAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA5410.10986600814754943No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5400.10966292865004934No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5390.10945984915254926No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT5370.10905369015754907No Hit
CCTCACCATACTGCACTAAGGCAAGCAATTCTGTGCTGGGGGGACCTAAT5300.10763213367504844No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5260.10681981568504807No Hit
CTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC5230.1062105771925478No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTTGGACGGAAATTGCACC5200.10560133870004752No Hit
GAAACGATGTATATTTGCGGGAAAGAACAACAGAGTTATCCGTCCCGAGA5200.10560133870004752No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5200.10560133870004752No Hit
CTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTAT5170.10499210020754725No Hit
GTTCAGCGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGATA5140.10438286171504697No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG5110.1037736232225467No Hit
GAATACATGCATATAATGGCATTAAAGCAGGGTAACCACATTGTGTGAAA5110.1037736232225467No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5080.10316438473004642No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG5070.10296130523254633No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5050.10255514623754615No Hit
ATCTCAATCTCGGGAATCTTAATGTTAGTATTCCTTGGACTCATAAGGTG5040.10235206674004606No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAA5010.10174282824754578No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT4950.10052435126254523No Hit
CTCCCCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4930.10011819226754505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)