FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493400
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA31690.6422780705310094No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26540.5379002837454399No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT23160.46939602756384274No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14790.29975678962302393No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14340.29063640048642075No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA13560.2748277259829753No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT12170.24665585731657885No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA10760.21807863802188895No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9550.19355492501013377No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9430.19112282124037291No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9370.1899067693554925No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9230.18706931495743817No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9190.18625861370085126No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9160.18565058775841103No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9130.1850425618159708No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8800.1783542764491285No Hit
CATTTGAACCCTACCAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8620.17470612079448722No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC8620.17470612079448722No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACCACACATAGCGCC8490.17207134171057964No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8480.17186866639643292No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTT8420.1706526145115525No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC8280.16781516011349817No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8260.1674098094852047No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT7680.15565464126469397No Hit
ACTGTGCAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7680.15565464126469397No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7580.15362788812322659No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7530.1526145115524929No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG7520.15241183623834617No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6930.1404539927036887No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6770.1372111876773409No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6770.1372111876773409No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTT6760.13700851236319417No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6550.13275233076611268No Hit
ATATAAGAGAGAAACCACACATAGCGCCTCATTTTGTGGGTCACCATATT6510.13194162950952573No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6290.12748277259829752No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6150.1246453182002432No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6050.12261856505877584No Hit
ATATTAGTGTTGACATAACTGACTATTAGGTCCCTGGATGTTGGATCTTC6040.12241588974462911No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTT5980.12119983785974868No Hit
ATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC5900.11957843534657478No Hit
GCTCTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT5670.11491690312119983No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5660.1147142278070531No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5650.11451155249290637No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5600.11349817592217266No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5580.1130928252938792No Hit
GTTTAGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA5580.1130928252938792No Hit
GAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTTTTATCCAAAGGTTA5530.11207944872314553No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5400.10944466963923793No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTTTTATCC5340.1082286177543575No Hit
GCTTAGCCTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT5250.1064045399270369No Hit
ATATTCATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGC5210.10559383867044994No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA5180.10498581272800973No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTCTATTCTTCTACTGTACCTGTC5120.10376976084312932No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGGTAGGGTTCAAATGTATAC5060.10255370895824888No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5020.10174300770166193No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5000.10133765707336846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)