FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493400
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA32550.6597081475476287No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26430.5356708552898257No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT21780.441426834211593No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14730.2985407377381435No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12930.26205918119173083No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA12890.26124847993514394No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT12220.24766923388731252No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC11450.23206323469801377No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11020.2233481961897041No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG10930.22152411836238345No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA10710.21706526145115526No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10640.2156465342521281No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10040.20348601540332387No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9620.1949736522091609No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9490.19233887312525333No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9110.18463721118767734No Hit
ACTGTGCAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8950.18139440616132954No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT8650.17531414673692744No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8640.17511147142278072No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8510.17247669233887314No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACCACACATAGCGCC8180.1657884069720308No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7980.16173490068909607No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7770.1574787190920146No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTT7510.15220916092419942No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC7490.15180381029590598No Hit
CATTTGAACCCTACCAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7200.1459262261856506No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6740.1366031617349007No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6700.13579246047831373No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6450.13072557762464532No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6440.13052290231049857No Hit
GTTTAGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA6370.12910417511147143No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG6220.12606404539927035No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTT6100.12363194162950952No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6040.12241588974462911No Hit
ATATAAGAGAGAAACCACACATAGCGCCTCATTTTGTGGGTCACCATATT5990.12140251317389543No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5960.12079448723145521No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5850.1185650587758411No Hit
ATATTAGTGTTGACATAACTGACTATTAGGTCCCTGGATGTTGGATCTTC5810.11775435751925414No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5800.11755168220510742No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5690.11532225374949331No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5620.11390352655046615No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA5550.11248479935143899No Hit
GCTCTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT5510.11167409809485206No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5450.11045804620997163No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTT5410.10964734495338467No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5410.10964734495338467No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA5350.10843129306850426No Hit
CCTTTGGATAAAACCTAGCAGGCATGATCAATTGCAGTCTTCTTTTCTCA5280.10701256586947709No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5070.10275638427239561No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5060.10255370895824888No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5010.1015403323875152No Hit
GAAAAGAAGACTGCAATTGATCATGCCTGCTAGGTTTTATCCAAAGGTTA4980.100932306445075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)