FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480087
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27240.5673971592648832No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA23730.49428541076929805No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGTCCACT14250.2968212011572902No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12490.2601611791196179No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12200.25412060730659236No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11020.2295417288949711No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG11010.22933343331521164No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAAC10890.2268338863580976No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10760.22412604382122406No Hit
CACTAATATGGGCCTAAAGTTTCGGCAACTATTGTGGTTTCACATTTCTT10670.22225138360338856No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10370.2160025162106035No Hit
GGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTG10170.21183660461541348No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC10050.20933705765829944No Hit
GCTTAGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG9710.20225500794647638No Hit
CCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9530.19850568751080536No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9520.19829739193104584No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC9290.1935065935965773No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA8650.18017567649196917No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC8620.17955078975269065No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8320.1733019223599056No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8100.16871941960519657No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8040.16746964612663956No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT7940.16538669032904452No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7900.1645535080100065No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATT7720.16080418757433548No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7650.15934611851601896No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA7530.15684657155890494No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7430.15476361576130993No Hit
GTATGCATGTATTCAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACA7400.15413872902203143No Hit
GGGTAAAGGGTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7290.1518474776446769No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7130.14851474836852488No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7110.14809815720900588No Hit
CATGCATACAGAGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGGGC6770.1410161074971828No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA6660.13872485611982827No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT6650.13851656054006878No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG6600.13747508264127126No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6570.13685019590199277No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6480.13497553568415724No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA6470.13476724010439775No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6460.13455894452463824No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6390.13310087546632174No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6330.1318511019877647No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6260.1303930329294482No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC6200.1291432594508912No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6170.1285183727116127No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT6140.1278934859723342No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTA6050.12601882575449866No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6040.12581053017473917No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5900.12289439205810614No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGATAGAACAACAGAGT5890.12268609647834663No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5840.12164461857954914No Hit
TAATAACTCTATCCACCTGGGTGGGTGGTAATTTGGAAGATCCAATATCC5800.12081143626051112No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5680.11831188930339709No Hit
CTAATAACTCTATCCACCTGGGTGGGTGGTAATTTGGAAGATCCAATATC5630.11727041140459958No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAA5550.11560404676652357No Hit
GTCCTAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGG5540.11539575118676407No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGACCTAC5500.11456256886772606No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5500.11456256886772606No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5480.11414597770820706No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5450.11352109096892855No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTA5400.11247961307013105No Hit
GCTTTAATGCCTCTGTATGCATGTATTCAATCTAAACAGGCTTTCACTTT5380.11206302191061203No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5330.11102154401181452No Hit
ATCCAAATGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT5250.10935517937373852No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5230.1089385882142195No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5230.1089385882142195No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5220.10873029263446No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5220.10873029263446No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5170.1076888147356625No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5120.10664733683686499No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA5100.10623074567734597No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA5030.10477267661902948No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5020.10456438103926997No Hit
CACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGT5010.10435608545951046No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4960.10331460756071295No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCATTGAC4940.10289801640119395No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGATCCTTAT4930.10268972082143446No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4900.10206483408215594No Hit
CTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA4850.10102335618335843No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA4840.10081506060359893No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4840.10081506060359893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)