FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380510
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT14890.3913169167695987No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12210.3208851278547213No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12080.3174686604819847No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT11840.31116133610154795No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10240.26911250689863603No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9170.24099235236918873No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC8620.22653806733068776No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8600.22601245696565134No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8450.2220703792278784No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT8290.2178654963075872No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8150.2141862237523324No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA8070.21208378229218683No Hit
TTACATATCCCATAAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA7970.20945573046700483No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA7170.18843131586554887No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTGAGTACAATA6900.18133557593755747No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6780.1781819137473391No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6660.1750282515571207No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6620.1739770308270479No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6280.16504165462142914No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6270.16477884943891094No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT6100.16031116133610157No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5860.15400383695566477No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5710.15006175921789178No Hit
TTGACATACTTTCCAATCAATCGGTCTATTTACAGGCAATTTTCGAAAAC5680.14927334367033718No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5670.14901053848781898No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA5520.14506846075004598No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5390.1416519933773094No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCCACTG5360.1408635778297548No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA5350.1406007726472366No Hit
GAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5120.13455625344931801No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA5090.13376783790176341No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATTTACAT4980.13087698089406324No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4900.12877453943391765No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4830.12693490315629025No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATT4820.12667209797377205No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA4700.12351843578355365No Hit
ATTTATGCCTACAGCCTCCTAGTACATAAATCTTTAACCTAATCTCCTCC4610.12115318914088986No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4610.12115318914088986No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4560.11983916322829886No Hit
CTTTAGGACATGGACATTGACACCTATAAAGAATTTGGAGCATCTGTGGA4520.11878794249822608No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTC4490.11799952695067148No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4480.11773672176815327No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4440.11668550103808048No Hit
CTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4420.11615989067304408No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACATAAATCTTTAACCTAATCT4400.11563428030800767No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT4350.11432025439541667No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG4260.11195500775275288No Hit
CCTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCT4220.11090378702268008No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4210.11064098184016188No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4190.11011537147512548No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4160.10932695592757088No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC4140.10880134556253448No Hit
TCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4100.1077501248324617No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATCGGTCTAT4100.1077501248324617No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT4100.1077501248324617No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4050.1064360989198707No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG4050.1064360989198707No Hit
GATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATT4040.1061732937373525No Hit
GTAAATAGACCGATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT3820.10039157972195213No Hit
CTTTCCAATCAATCGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG3810.10012877453943392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)