FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380510
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14680.38579800793671654No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT14030.36871567107303355No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12040.31641743975191194No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT10000.2628051825181993No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9880.25965152032798083No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT9680.2543954166776169No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9200.24178076791674333No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT9180.2412551575517069No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC8870.23310819689364273No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7980.209718535649523No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7760.20393682163412263No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA7590.19946913353131326No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTGAGTACAATA7550.19841791280124044No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA7340.19289900396835827No Hit
TTACATATCCCATAAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA6940.18238679666763027No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6830.1794959396599301No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6770.1779191085648209No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6730.1768678878347481No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6560.1724001997319387No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6560.1724001997319387No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6330.16635568053402014No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6300.16556726498646554No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT5970.15689469396336497No Hit
GAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5900.15505505768573757No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5570.14638248666263698No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5370.14112638301227298No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5320.13981235709968198No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5260.1382355260045728No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA5050.13271661717169062No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5040.13245381198917242No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATT5010.13166539644161784No Hit
TTGACATACTTTCCAATCAATCGGTCTATTTACAGGCAATTTTCGAAAAC4910.12903734461643585No Hit
CTTTAGGACATGGACATTGACACCTATAAAGAATTTGGAGCATCTGTGGA4910.12903734461643585No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG4810.12640929279125385No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCCACTG4760.12509526687866285No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4610.12115318914088986No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4550.11957635804578066No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA4540.11931355286326245No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC4380.11510866994297127No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4370.11484586476045308No Hit
GTAAATAGACCGATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT4340.11405744921289848No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTC4250.1116922025702347No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4250.1116922025702347No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATTTACAT4180.10985256629260728No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAT4180.10985256629260728No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4150.1090641507450527No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA4090.1074873196499435No Hit
CTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4030.10591048855483429No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATCGGTCTAT4010.10538487818979791No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA3990.1048592678247615No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3960.10407085227720692No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT3870.1017056056345431No Hit
CTCTAAGGCCTCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAA3860.10144280045202492No Hit
CCTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCT3850.10117999526950672No Hit
ATTTATGCCTACAGCCTCCTAGTACATAAATCTTTAACCTAATCTCCTCC3840.1009171900869885No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA3810.10012877453943392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)