FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464433
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT22090.4756337297306608No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17230.3709900028637069No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13590.2926148658687044No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG13480.2902463864540203No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13430.2891698049018911No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTTGTAGAGATGGGAAAAAAT13410.28873917228103946No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGGAT12830.27625082627634123No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT12800.2756048773450638No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA12390.26677690861760467No Hit
CTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA11060.23813983933096916No Hit
TTCTAAAACAGTCTTTGATTTTCATTTTGATGTGTTCCTGTGGTAATGAC10930.23534072729543337No Hit
ATACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACATTTCT10790.2323262989494717No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTAAG10620.22866592167223257No Hit
CTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC10580.22780465643052927No Hit
CTATTTACATACCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTA10110.2176847898405152No Hit
ACATTATCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTT10000.21531631042583108No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT9860.21230188207986941No Hit
CTCCAAAAGACACCAAATACTCTATGACGGTTTCTCTTCCAAAAGTGAGA9810.21122530052774027No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9690.20864150480263027No Hit
GGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTTGTAGAGAT9480.20411986228368786No Hit
ATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCA9120.19636847510835792No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC9020.19421531200409964No Hit
CTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT8880.19120088365813798No Hit
ATTTTGTAGAGATGGGAAAAAATCTAGCAGGCATGACTAAGTTTAATCTT8820.189908985795583No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGGATA8560.1843107617245114No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8520.18344949648280806No Hit
CTCTATGACGGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8410.18108101706812393No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGA8280.17828190503258812No Hit
GTACGGGACCCTGCCGAACCTGCACGACTCTTGCTCAAGGAACCTCTATG8170.175913425617904No Hit
CCTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8060.17354494620321984No Hit
TTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAA7920.1705305178572582No Hit
GCCTAGCAGCCATGGAAAGGATGTATATTTCCGGGAGAGAACGACAGAAT7880.16966925261555488No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAATTTTCTT7780.16751608951129657No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTCTA7660.1649322937861866No Hit
CCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTACACGTAGCGCC7590.16342507961320576No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGG7530.1621331817506508No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC7460.16062596757766998No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC7280.15675027399000502No Hit
TTCTTGGACGGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC7240.1558890087483017No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7160.15416647826489505No Hit
CTTTTACACAATGTGGGTATCCTGCTTTAATGCCTCTGTATGCGTGTATT7140.15373584564404338No Hit
GCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAG7060.15201331516063674No Hit
GTTTTAGAAAACTCCCTGTTAACCGGCCTATTGATTGGAAAGTATGTCAA7020.1511520499189334No Hit
CTGTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGC6960.14986015205637843No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT6690.14404661167488098No Hit
GCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCAT6460.13909433653508688No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6450.13887902022466103No Hit
GTATCGGGATGCCTTAGAATCTCCTGAGCATTGTTCACCGCACCACACTG6370.13715648974125438No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGG6330.13629522449955106No Hit
GTCCAAGAAAGCCCCATGATGTAGCTCTTGTTCCCAAGAATAAGGTGACC6320.13607990818912521No Hit
GGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCT6250.1345726940161444No Hit
CTCCTATGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGC6240.13435737770571857No Hit
TATTAGTATTGACATAACTGACTACTAGGTCCCTGGATGCTGGATCTTCC6180.1330654798431636No Hit
GTCCCGTACTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6180.1330654798431636No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCG6080.1309123167389053No Hit
CCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAAATGAATATC6050.1302663678076278No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6040.13005105149720197No Hit
GTTATGTCAATACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTT6000.12918978625549865No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5980.128759153634647No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5820.12531409266783367No Hit
GTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5770.12423751111570454No Hit
GTATAGTAGAAGAGTAAAGCCCCGTAAAATTTCCCACCTTGTGAGTCCAA5740.12359156218442703No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5730.12337624587400119No Hit
GCATAGGAGCCACAAAGGTTCCGCGCATGCGCTGATGGCCTATGGCCAAG5680.12229966432187206No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCTTACTGAAGGAAA5610.12079245014889123No Hit
GCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGCAGGCTTTCACTTT5530.11906991966548458No Hit
GGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATG5490.11820865442378126No Hit
ATGTGGGTATCCTGCTTTAATGCCTCTGTATGCGTGTATTCAATCTAAGC5420.11670144025080044No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5390.11605549131952293No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAATCTCCTGA5390.11605549131952293No Hit
CTTTTATATAAGATGCCCGCCTTCCATAGGGTATGTAAATAGTGTCTGGT5370.11562485869867128No Hit
CTACAAAATATTTGCCCCTAGAGAAAGGTATAAAACCTTATTATCCAGAT5360.11540954238824545No Hit
GTACAACATCTTGAGTCCCTTTATACCTCTGTTACCAATTTTCTTTTGTC5360.11540954238824545No Hit
GGGTAAAGGTTCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5330.11476359345696796No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5260.11325637928398716No Hit
GTATGGATCGGCATAGGAGCCACAAAGGTTCCGCGCATGCGCTGATGGCC5240.11282574666313548No Hit
CATTTAAATCCCAACAAAACAAAAAGATGGGGATATTCCCTACATTTCAT5230.11261043035270964No Hit
CCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTT5190.11174916511100631No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5190.11174916511100631No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGTGATTGGGGTAGGCTGCCT5180.11153384880058048No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTATGA5140.11067258355887716No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCC5020.10808878783376719No Hit
ATGTAGCTCTTGTTCCCAAGAATAAGGTGACCCACAAAATGAGGCGCTAC4990.1074428389024897No Hit
TACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACATTTCTT4960.10679688997121221No Hit
GTTAGAGGACCTACAAATTGTTCACATTTGTTAATAATGTCCTGGTGCAA4950.10658157366078638No Hit
TAACAAATGTGAACAATTTGTAGGTCCTCTAACAGTAAATGAAAAACGAA4930.10615094103993471No Hit
CTACATTATCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATAT4930.10615094103993471No Hit
GTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCA4910.10572030841908306No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA4880.10507435948780557No Hit
ATCTAGAAGATCTCTTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4870.10485904317737973No Hit
TGTCAATACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACA4830.10399777793567641No Hit
GTATAAAACCTTATTATCCAGATAATGTAGTTAATCATTACTTCCAAACC4720.10162929852099226No Hit
GTCTACGTCCCGTCAGCGCTGAATCCTGCGGACGACCCGTCTCGGGGTCG4720.10162929852099226No Hit
CCTCTATGTTTCCCTCATGTTGCTGTTCAAAACCTTCGGACGGAAATTGC4720.10162929852099226No Hit
GAATTATCTGTCCCGATAAGTTTCGCTCCAGACCTGCTGCGAGCAAAACA4710.10141398221056644No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC4700.10119866590014061No Hit
CCAAATACTCTATGACGGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA4670.10055271696886312No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4650.10012208434801145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)