FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552783
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21920.39653896737055955No Hit
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGG20260.36650910031603723No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT18780.3397354839059812No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT14770.26719345565981584No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT14500.26230907969311645No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG14270.25814831498074287No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13860.25073129962390306No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTAAAGTTTAATCTA13100.2369826857917121No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACG12750.23065108731636103No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12430.22486219728175433No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAAA11570.2093045553137488No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGCTGACATACT11530.20858094405942296No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT10370.18759621768397364No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9920.17945559107280795No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC9910.1792746882592265No Hit
CTCTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG9900.17909378544564503No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGTGTTCACGGTGGTCTCCATGC9890.17891288263206356No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9710.17565663198759732No Hit
TATCCATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAAA9670.17493302073327147No Hit
ATTTATAGGAAGTTTCCGAAAACAATGTTTGATTTTGTGTACAATATGTT9600.17366670103820125No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG9320.16860142225792038No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9270.16769690819001307No Hit
CATGTATACAAGCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAAACC9160.16570697724061703No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9070.1640788519183839No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT9000.16281253222331366No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8980.16245072659615076No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG8780.15883267032452156No Hit
GGGTAAAGGTTGAGATATTGTTTACACAGAAAGGTTTTGTAAGTTGGCGA8730.15792815625661427No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8620.1559382253072182No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACCTTGCCACAA8590.15539551686647382No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATG8460.15304378028991486No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8370.15141565496768172No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8120.14689308462814524No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAT7910.14309412554293457No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7650.13839065238981663No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7500.13567711018609474No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7490.13549620737251328No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7410.13404898486386158No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT7410.13404898486386158No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA7400.1338680820502801No Hit
CTCCAAACGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7380.13350627642311721No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7230.1307927342193953No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7210.1304309285922324No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7180.12988822015148802No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA7150.12934551171074363No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT7060.12771738638851052No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT6970.12608926106627735No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6950.12572745543911443No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6920.12518474699837007No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATGGAAGGAAA6820.12337571886255547No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGTGTTC6740.1219284963539038No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6690.12102398228599649No Hit
GCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTT6620.11975766259092627No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6620.11975766259092627No Hit
CAATCCGCTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTC6540.1183104400822746No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6520.11794863445511168No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6510.11776773164153022No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6370.11523509225138978No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6330.11451148099706394No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6320.11433057818348248No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6290.11378786974273812No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6270.1134260641155752No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6260.11324516130199373No Hit
GAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCA6250.11306425848841227No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA6230.11270245286124936No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACAATGTTTGATTTTGTGTACAAT6200.11215974442050497No Hit
TTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTA6190.11197884160692351No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAGCGAATTGTGGGGCT6160.11143613316617912No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6160.11143613316617912No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6120.11071252191185331No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCGTCTCG6110.11053161909827185No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA6100.11035071628469038No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6080.10998891065752746No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6040.10926529940320162No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5960.10781807689454995No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5950.10763717408096848No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCAAAGCAGGCTTTCACTTT5950.10763717408096848No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC5900.10673266001306118No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5900.10673266001306118No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5760.10420002062292075No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5760.10420002062292075No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5700.10311460374143198No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5700.10311460374143198No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5690.10293370092785052No Hit
GGTACGGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTT5570.10076286716487301No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5560.10058196435129155No Hit
GCCTAGAAGCCCCACAATTCGCTGACATACTTTCCAATCAATAGGTCTAT5550.10040106153771008No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5530.10003925591054717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)