FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470193
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA33350.7092832092353565No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20490.43577849946724007No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16890.35921419502204416No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14060.29902614458318183No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12440.26457220758284367No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11910.2533002405395231No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11860.25223684742222874No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11460.24372970248387363No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10920.23224505681709423No Hit
CCCTTATCCAATGGCAAATATTTTGTAACATTTGGATAAAACCTAGCAGG10370.220547732526856No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10330.21969701803302047No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTTGTAACATTTGGATAAAAC10300.21905898216264386No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10080.21438005244654854No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA9710.20651094337857007No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9640.20502219301435795No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA9270.19715308394637945No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT9120.1939629045944963No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8900.18928397487840098No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8760.18630647414997673No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8670.18439236653884683No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8560.18205290168079916No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTTGTAACATT8250.17545986435357397No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT8010.1703555773905609No Hit
ATATTAGTGTTAACATAACTGACTACGAGGTCCCTGGATATTGGATCTTC7980.16971754152018428No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7740.16461325455717118No Hit
AAACAAAAAGGTGGGGTTACTCTTTACATTTTATGGGCTATGTCATTGGA7700.1637625400633357No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7610.1618484324522058No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG7570.16099771795837028No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7570.16099771795837028No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7530.16014700346453478No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7500.15950896759415814No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT7440.1582328958534049No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7440.1582328958534049No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7380.1569568241126516No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7260.1544046806311451No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7060.15015110816196753No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7030.14951307229159091No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6850.14568485706933113No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6790.14440878532857784No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6590.1401552128594003No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6440.13696503350751713No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6400.13611431901368162No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6300.13398753277909284No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA6290.13377485415563398No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT6260.13313681828525734No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6250.13292413966179845No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6120.13015931755683305No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6100.1297339603099153No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6030.12824520994570315No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6010.12781985269878537No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5870.12484235197036112No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5860.12462967334690223No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5820.12377895885306671No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5800.12335360160614897No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5770.12271556573577233No Hit
TAATAAATCTATCCACCTGGGTGGGTGGTAATTTGGAAGATCCAATATCC5710.12143949399501908No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5610.11931270776043029No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5570.11846199326659478No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5560.1182493146431359No Hit
CATTTAAACCCTAACAAAACGAAAAGGTGGGGTTACTCTTTACATTTTAT5530.11761127877275927No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5520.1173986001493004No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5500.11697324290238263No Hit
TTTTCGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTG5450.11590984978508825No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5440.11569717116162938No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5390.11463377804433499No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5370.11420842079741725No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5320.11314502768012284No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5250.1116562773159107No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5250.1116562773159107No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5250.1116562773159107No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT5250.1116562773159107No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5220.11101824144553406No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5220.11101824144553406No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT5200.1105928841986163No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5190.11038020557515744No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5180.11016752695169857No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT5120.10889145521094529No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5040.10719002622327427No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4970.10570127585906212No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTTGTAACATTTGGATAAAA4950.10527591861214437No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4950.10527591861214437No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTTGTA4910.10442520411830887No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG4910.10442520411830887No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4870.10357448962447335No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4820.10251109650717898No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACAAAATATTTGCCATTGG4810.10229841788372009No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4790.10187306063680233No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC4740.10080966751950796No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4740.10080966751950796No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT4730.10059698889604907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)