FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542200
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32210.5940612320177057No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC30440.5614164514939137No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA26200.483216525267429No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT18080.33345628919218No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC17550.3236812984138694No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC17320.31943932128365915No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA16000.2950940612320177No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG13650.2517521209885651No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13400.24714127628181481No Hit
CCCTCATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATG12510.23072666912578385No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAAT12450.22962006639616378No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT12100.2231648838067134No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12000.22132054592401326No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT11760.216894135005533No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA11190.20638140907414237No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA10960.20213943194393214No Hit
AAATCGACCTATTGATTACAAAGTATGTCAAAGAATTGTGGGTCTTTTGG10950.2019549981556621No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10900.20103282921431204No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10280.18959793434157138No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9920.18295831796385098No Hit
GTTTTAAAATAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9380.1729988933972704No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9310.1717078568793803No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT9160.16894135005533015No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC9040.16672814459609No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8890.16396163777203984No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8810.16248616746587977No Hit
AAATAGACCTATTGATTACAAAGTATGTCAAAGAATTGTGGGTCTTTTGG8800.16230173367760972No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8690.16027296200663962No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT8630.15916635927701955No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8480.1563998524529694No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8450.15584655108815934No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC8430.15547768351161934No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG8040.1482847657690889No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7940.14644042788638878No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7780.1434894872740686No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG7760.1431206196975286No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT7750.14293618590925858No Hit
CCGATGGCCTATGGCCAAGCCCCAGCCAGTGGGGGTTGCATCAGCAAACA7600.14016967908520842No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7540.13906307635558834No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG7450.13740317226115825No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7380.13611213574326816No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7370.13592770195499815No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7360.13574326816672813No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7310.1348210992253781No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7110.13113242345997786No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG7000.12910365178900773No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6960.1283659166359277No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6840.12615271117668755No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6830.12596827738841757No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT6710.12375507192917742No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6660.12283290298782737No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6640.12246403541128735No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6580.12135743268166727No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6540.12061969752858723No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6480.11951309479896717No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC6470.11932866101069717No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCT6460.11914422722242715No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA6450.11895979343415715No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6440.11877535964588712No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT6370.11748432312799705No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6360.11729988933972704No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA6350.11711545555145703No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6330.11674658797491701No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6300.11619328661010696No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG6290.11600885282183696No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6240.1150866838804869No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6220.11471781630394688No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6080.11213574326816673No Hit
GCCTTTCTGTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACG6080.11213574326816673No Hit
TCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6050.1115824419033567No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6030.11121357432681667No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGATCTCCTCGACACC6000.11066027296200663No Hit
CCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATG5990.11047583917373663No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5960.1099225378089266No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5950.10973810402065659No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA5850.10789376613795647No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5830.10752489856141645No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC5830.10752489856141645No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5810.10715603098487643No Hit
GTTTTGAAATAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5800.10697159719660643No Hit
GCACACCCGAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAAC5750.10604942825525636No Hit
ATTTCAAAACTAGGCATTATTTACATACTCTGTGGAAGGCGGGCATTCTA5720.10549612689044632No Hit
GGCCCTCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATAC5670.10457395794909628No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT5650.10420509037255626No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5620.10365178900774623No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA5620.10365178900774623No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5610.1034673552194762No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA5510.1016230173367761No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5500.1014385835485061No Hit
GCATTGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC5500.1014385835485061No Hit
GAGCAAAACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATAC5470.10088528218369604No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5450.10051641460715602No Hit
GGGCCATTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTT5430.100147547030616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)