FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542200
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32100.5920324603467355No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA27260.5027665068240501No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC26180.48284765769088894No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC17430.3214680929546293No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT15740.29029878273699744No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA14980.2762818148284766No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13950.2572851346366654No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAAT13020.2401327923275544No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC12120.22353375138325343No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG11620.21431206196975286No Hit
CCCTCATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATG11430.21080781999262263No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT10930.20158613057912209No Hit
AAATCGACCTATTGATTACAAAGTATGTCAAAGAATTGTGGGTCTTTTGG10820.19955735890815196No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT10800.19918849133161196No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA10430.19236444116562154No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA10260.18922906676503135No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10000.18443378827001106No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG9820.18111398008115087No Hit
GTTTTAAAATAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9480.17484323127997048No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9380.1729988933972704No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9180.16931021763187015No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC9160.16894135005533015No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG8990.16580597565473995No Hit
AAATAGACCTATTGATTACAAAGTATGTCAAAGAATTGTGGGTCTTTTGG8920.16451493913684986No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8890.16396163777203984No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG8810.16248616746587977No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8750.16137956473625967No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG8660.15971966064182958No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8510.1569531538177794No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8370.15437108078199926No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8080.14902250092216893No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA8050.1484691995573589No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7780.1434894872740686No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT7690.1418295831796385No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC7590.1399852452969384No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT7580.13980081150866838No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7530.13887864256731833No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT7520.1386942087790483No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7430.13703430468461822No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7220.13316119513094798No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG7200.13279232755440798No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7130.1315012910365179No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7130.1315012910365179No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7120.13131685724824788No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7050.13002582073035782No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6940.1279970490593877No Hit
CCGATGGCCTATGGCCAAGCCCCAGCCAGTGGGGGTTGCATCAGCAAACA6900.12725931390630765No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6840.12615271117668755No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6820.12578384360014755No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6360.11729988933972704No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6350.11711545555145703No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6300.11619328661010696No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC6270.11563998524529694No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6250.11527111766875692No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGATCTCCTCGACACC6230.1149022500922169No Hit
GCCTTTCTGTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACG6200.11434894872740686No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT6130.11305791220951678No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6110.11268904463297676No Hit
GGCCCTCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATAC6110.11268904463297676No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6080.11213574326816673No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA6060.11176687569162672No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6050.1115824419033567No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6020.11102914053854665No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6010.11084470675027665No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6000.11066027296200663No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5950.10973810402065659No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5900.10881593507930654No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5900.10881593507930654No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5890.1086315012910365No Hit
GAGCAAAACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATAC5880.1084470675027665No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5870.10826263371449649No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5850.10789376613795647No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5830.10752489856141645No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT5810.10715603098487643No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT5800.10697159719660643No Hit
CGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC5790.10678716340833641No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC5730.10568056067871634No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5710.10531169310217632No Hit
GTTTTGAAATAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5700.1051272593139063No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG5700.1051272593139063No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA5670.10457395794909628No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTC5550.10236075248985614No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5520.1018074511250461No Hit
GCATTGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC5490.10125414976023608No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5460.10070084839542603No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCT5440.10033198081888602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)