FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358237
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32720.9133618247138068No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC20730.578667195180844No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17260.48180394543277216No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16200.45221459536563785No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC11640.3249245611145694No Hit
CCTAGTATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTT11170.31180475495272686No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC10710.2989640936028383No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT10090.2816571152616843No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9480.26462928173248434No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8950.2498346066989172No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTATCTCCTCC8610.2403436830924779No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8070.22526986324695664No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTG8020.22387413918718613No Hit
GGTCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCAACCACGGGGCG7910.2208035462556911No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7830.21857038776005827No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT7700.21494150520465502No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGACGAGAAGGCACAG7700.21494150520465502No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCC7620.21270834670902225No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7540.21047518821338948No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7440.2076837400938485No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA7150.19958854054717964No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT6930.19344735468418953No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6550.1828398518299338No Hit
GTCCGGCAGACGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6410.17893182446257647No Hit
ACCCATCTCGGGGCCGTTTGGGTCTCTACCGTCCCCTTCTTCATCTGCCG6230.1739072178474027No Hit
GTAGAGACCCAAACGGCCCCGAGATGGGTCGTCCGCGGGATTCAGCGCCG6100.17027833529199943No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACT6090.16999919048004533No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG6090.16999919048004533No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5970.16664945273659615No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5900.1646954390529175No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG5880.16413714942900928No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5840.1630205701811929No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG5640.15743767394211095No Hit
CTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTGCACTTCGCTTCAC5600.15632109469429456No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCGGTCTTACATAAGAGGACTCT5540.15464622582256998No Hit
TTCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5460.15241306732693718No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5350.14934247439544213No Hit
GGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG5280.14738846071176342No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGG5260.14683017108785523No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5250.14655102627590116No Hit
GTCCAAGAGTCCTCTTATGTAAGACCGAGAGTCCAAGAGTCCTCTTATGT5240.14627188146394704No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA5160.14403872296831427No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5080.1418055644726815No Hit
GTGCACACGGTCCGGCAGACGAGAAGGCACAGACGGGGAGACCGCGTAAA5030.14040984041291102No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5020.1401306956009569No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4970.1387349715411864No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTA4960.13845582672923232No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4930.13761839229337003No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4920.13733924748141593No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4910.13706010266946184No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGACGAGAAGGCACAGACGGGGA4900.13678095785750774No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4850.13538523379773723No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCTTAT4840.13510608898578316No Hit
ACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGC4780.13343122011405859No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT4730.13203549605428808No Hit
GTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4720.13175635124233398No Hit
ATGTAAGACCGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGA4720.13175635124233398No Hit
AGTATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAA4700.1311980616184258No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT4690.1309189168064717No Hit
TCTCGGGGCCGTTTGGGTCTCTACCGTCCCCTTCTTCATCTGCCGTTCCG4650.1298023375586553No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4530.12645259981520612No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4470.12477773094348155No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT4440.12394029650761924No Hit
CTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGA4420.12338200688371105No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG4360.12170713801198647No Hit
GATCCATACTGCGGAACTCCTAGCTGCTTGTTTTGCTCGCAGCCGGTCTG4360.12170713801198647No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC4320.12059055876417009No Hit
CTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA4320.12059055876417009No Hit
GGTTGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC4310.12031141395221598No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGT4220.11779911064462913No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4170.11640338658485863No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4150.11584509696095044No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4120.11500766252508814No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGGCAGATGAAGAAGG4120.11500766252508814No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC4100.11444937290117994No Hit
CTACTGTACCTGTCTTTAATTCTGAGTGGCAAACTCCCTCCTTTCCTCAC4040.11277450402945535No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3990.11137877996968487No Hit
GACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC3990.11137877996968487No Hit
GTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTGCACTTCGCTTC3970.11082049034577668No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3960.11054134553382258No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCTAGCAGCC3940.10998305590991439No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3890.1085873318501439No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCAACCACGGGGCGCACCT3860.10774989741428159No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3860.10774989741428159No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3850.10747075260232751No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3840.1071916077903734No Hit
GGATAATAAGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT3830.10691246297841932No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3770.10523759410669474No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT3720.10384187004692425No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT3660.10216700117519967No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC3640.10160871155129148No Hit
ATACTGCGGAACTCCTAGCTGCTTGTTTTGCTCGCAGCCGGTCTGGAGCA3630.10132956673933737No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3590.10021298749152098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)