FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358237
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32640.911128666218174No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15770.4402113684516117No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15310.4273707071017232No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC13860.38689470936837905No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT11630.3246454163026153No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTG10830.30231383134628753No Hit
GGTCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCAACCACGGGGCG9520.2657458609803008No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC9260.2584880958694942No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA9130.25485921331409095No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8960.25011375151087134No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8850.24704315857937623No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA8770.24481000008374346No Hit
CTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTGCACTTCGCTTCAC8620.24062282790443196No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTATCTCCTCC8600.2400645382805238No Hit
ACCCATCTCGGGGCCGTTTGGGTCTCTACCGTCCCCTTCTTCATCTGCCG8510.23755223497293693No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCC8030.22415328399914025No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7710.21522065001660912No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG7670.21410407076879273No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG7600.21215005708511403No Hit
TTCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7510.20963775377752716No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC7500.2093586089655731No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7400.2065671608460321No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7330.2046131471623534No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7270.20293827829062883No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6970.1945639339320059No Hit
GTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTGCACTTCGCTTC6310.17614037634303548No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACT6310.17614037634303548No Hit
GTAGAGACCCAAACGGCCCCGAGATGGGTCGTCCGCGGGATTCAGCGCCG6240.1741863626593568No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6160.171953204163724No Hit
TCTCGGGGCCGTTTGGGTCTCTACCGTCCCCTTCTTCATCTGCCGTTCCG6100.17027833529199943No Hit
CCTAGTATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTT6060.16916175604418304No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5950.16609116311268796No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5910.16497458386487157No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCGGTCTTACATAAGAGGACTCT5890.16441629424096338No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5800.1619039909333765No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5790.16162484612142242No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5700.15911254281383555No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5620.15687938431820275No Hit
CTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA5520.15408793619866176No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGACGAGAAGGCACAG5350.14934247439544213No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5290.14766760552371755No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCAACCACGGGGCGCACCT5280.14738846071176342No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG5260.14683017108785523No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA5160.14403872296831427No Hit
GGTTGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC5010.1398515507890028No Hit
CTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGA4980.1390141163531405No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4840.13510608898578316No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4830.13482694417382904No Hit
GATCCATACTGCGGAACTCCTAGCTGCTTGTTTTGCTCGCAGCCGGTCTG4710.13147720643037988No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4670.1303606271825635No Hit
GTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4660.1300814823706094No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG4650.1298023375586553No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCTTAT4550.12701088943911432No Hit
GTTTGGGTCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCAACCACG4460.12449858613152746No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT4430.12366115169566516No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4400.12282371725980286No Hit
CCTCTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGT4370.12198628282394058No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4370.12198628282394058No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4350.12142799320003239No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4350.12142799320003239No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4270.1191948347043996No Hit
GTCTGTGCCTTCTCGTCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGC4260.11891568989244551No Hit
GTCCAAGAGTCCTCTTATGTAAGACCGAGAGTCCAAGAGTCCTCTTATGT4240.11835740026853732No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4190.11696167620876682No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGG4120.11500766252508814No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4040.11277450402945535No Hit
GTCCGGCAGACGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3990.11137877996968487No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3910.10914562147405209No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC3900.108866476662098No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3880.10830818703818978No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG3870.1080290422262357No Hit
CTCTTGGACTCTCGGTCTTACATAAGAGGACTCTTGGACTCTCAGCAATG3860.10774989741428159No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3850.10747075260232751No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3820.10663331816646521No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3800.10607502854255701No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGGCAGATGAAGAAGG3790.10579588373060293No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA3780.10551673891864882No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3730.10412101485887833No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3690.10300443561106194No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT3680.10272529079910786No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3650.10188785636324556No Hit
ACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG3610.10077127711542917No Hit
GGATAATAAGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT3600.10049213230347509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)