FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531050
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21030.396007908859806No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17110.3221918840033895No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14880.2801996045570097No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12940.24366820450051782No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11230.2114678467187647No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11000.20713680444402596No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG10630.20016947556727235No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10500.19772149515111573No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9910.1866114301854816No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9840.18529328688447416No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG9730.1832219188400339No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC9710.18284530646831748No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA9700.1826570002824593No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8960.16872234252895207No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8830.16627436211279542No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8700.16382638169663874No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8590.1617550136521985No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8450.1591187270501836No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8420.158553808492609No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAA8020.15102156105828077No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA7900.1487618868279823No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA7880.14838527445626587No Hit
ATGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC7720.1453723754825346No Hit
GATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC7640.14386592599566894No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTTCGAGAGAGGACAACCGAGT7600.14311270125223613No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7590.1429243950663779No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGT7580.14273608888051972No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7560.14235947650880332No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7440.14009980227850485No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT7410.13953488372093023No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7330.13802843423406458No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7050.13275586103003484No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6940.1306844929855946No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6860.12917804349872894No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA6830.12861312494115432No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC6800.1280482063835797No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA6800.1280482063835797No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6790.12785990019772148No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6780.1276715940118633No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATG6650.12522361359570663No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6650.12522361359570663No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6540.12315224555126637No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6450.12145748987854252No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6380.12013934657753506No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6360.11976273420581866No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6100.11486677337350532No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6080.11449016100178891No Hit
GTGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC6060.11411354863007249No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6030.11354863007249788No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5910.11128895584219942No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT5870.11053573109876659No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5830.10978250635533376No Hit
GTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCTG5810.10940589398361737No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG5760.10846436305432634No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5700.1073345259391771No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5680.10695791356746069No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5650.10639299500988608No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5650.10639299500988608No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5640.10620468882402787No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5580.10507485170887863No Hit
GATTAAGATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT5540.10432162696544581No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGT5520.1039450145937294No Hit
ACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGG5510.1037567084078712No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5510.1037567084078712No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5510.1037567084078712No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAAT5510.1037567084078712No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5510.1037567084078712No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5500.10356840222201298No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5440.10243856510686376No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5440.10243856510686376No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5440.10243856510686376No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5430.10225025892100556No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5420.10206195273514734No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT5410.10187364654928915No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5390.10149703417757273No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5340.1005555032482817No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5320.1001788908765653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)