FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401870
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56631.4091621668698833No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG15320.3812178067534277No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11530.2869087018189962No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCTCTTAACTTTAT11320.28168313136088785No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTGAGCTTTTGTACAATATGTT9380.233408813795506No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG9210.22917859009132305No Hit
CTCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8920.22196232612536393No Hit
ATCCTAACCTTACCAAATATTTACCATTGGATAAAGGCATTAAACCTTAT8020.19956702416204244No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7060.17567870206783287No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6820.1697066215442805No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGCTTTTGTACAATA6810.16945778485579913No Hit
GGATAAAGGCATTAAACCTTATTATCCGGAACATGCAGTTAATCATTACT6810.16945778485579913No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCA6790.16896011147883644No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6690.16647174459402295No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6660.1657252345285789No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6570.16348570433224677No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6430.16000199069350785No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6170.15353223679299277No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA6130.15253689003906734No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5930.14756015626944036No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5530.13760668873018636No Hit
TGGGCATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCTCTT5470.1361136685992983No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC5340.13287879164904073No Hit
GGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTT5320.13238111827207805No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCC5190.12914624132182048No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCATTAAC5070.1261602010600443No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT5040.12541369099460026No Hit
CCTTTATCCAATGGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4880.12143230397889866No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4850.12068579391345459No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA4810.1196904471595292No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4670.1162067335207903No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4650.1157090601438276No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCGGCA4610.1147137133899022No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4580.11396720332445816No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4560.11346952994749546No Hit
ATATTTACCATTGGATAAAGGCATTAAACCTTATTATCCGGAACATGCAG4510.11222534650508871No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4420.10998581630875656No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4420.10998581630875656No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4390.10923930624331252No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4380.10899046955483117No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4360.10849279617786846No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4350.1082439594893871No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4320.10749744942394307No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC4260.10600442929305497No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4240.10550675591609227No Hit
ACATCAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG4180.10401373578520416No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4130.10276955234279743No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCCGGATAATAAGGTTTAATGCC4080.10152536890039067No Hit
GTTTTATCCTAACCTTACCAAATATTTACCATTGGATAAAGGCATTAAAC4060.10102769552342797No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4030.10028118545798394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)