FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476994
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24750.5188744512509591No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGCCTCCATGC13140.27547516320960014No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12700.2662507285206942No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG10280.2155163377317115No Hit
CTCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAG9960.20880765795796172No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9930.20817871922917272No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9650.20230862442714165No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9310.1951806521675325No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9210.1930841897382357No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8770.18385975504932978No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8560.17945718394780646No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC8050.16876522555839277No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7770.16289513075636172No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7600.15933114462655715No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7560.15849255965483844No Hit
CTCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT7540.15807326716897907No Hit
GTATACATGCGTATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA7470.1566057434684713No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7150.14989706369472153No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6970.1461234313219873No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6970.1461234313219873No Hit
CCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTC6710.1406726290058156No Hit
CCTCTGCACGTCGCATGGAGGCCACCGTGAACGCCCACCAGGTCTTGCCC6620.13878581281944846No Hit
CATGTATACAATCTAAGCAGGCTTTCTCTTTCTCGCCAACTTACAAGGCC6590.1381568740906594No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6510.136479704147222No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6390.1339639492320658No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC6360.13333501050327679No Hit
CGGTGGCCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6340.13291571801741742No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA6340.13291571801741742No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6310.13228677928862836No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTGAGGGAG6200.1299806706164019No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6070.12725526945831606No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6040.12662633072952698No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC6030.12641668448659732No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6020.12620703824366764No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACT5990.1255780995148786No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG5910.12390092957144114No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA5790.12138517465628498No Hit
GGCCATAGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATAC5750.12054658968456626No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG5740.12033694344163659No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG5720.11991765095577722No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA5650.11845012725526945No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5500.11530543361132424No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5480.1148861411254649No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG5470.1146764948825352No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5460.11446684863960553No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5450.11425720239667583No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA5310.11132215499566032No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5310.11132215499566032No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5300.11111250875273065No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5280.11069321626687129No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5240.10985463129515255No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5110.10712923013706671No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5090.10670993765120734No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5050.10587135267948863No Hit
CCTATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACA5000.10482312146484023No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4970.10419418273605119No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATACGCATGTATA4950.10377489025019182No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4820.10104948909210598No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA4770.10000125787745757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)