FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509108
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG22780.4474492642032732No Hit
GTTTCAATACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACA17610.34589910195872No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16740.32881038993690925No Hit
GTATTAAAACTCAAACAATGTTTTCGGAAATTGCCTGTAAATAGACCTAT16680.3276318580733361No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT16000.3142751636195071No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15920.3127037878014095No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14630.2873653527345868No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13870.2724372824626602No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13390.26300902755407496No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC12740.25024159903203247No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA12620.2478845353048862No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12500.2455274715777399No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11910.23393857491927056No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11780.23138508921486206No Hit
TTATTAATCGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11740.2305994013058133No Hit
GTTTTAATACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACA10920.21449279917031358No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10630.20879656182971No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10570.20761802996613685No Hit
CCCTCATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATG10210.20054683878469795No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA10090.19818977505755164No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10050.19740408714850288No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9900.19445775748957No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9870.19386849155778343No Hit
GTATTGAAACTCAAACAATGTTTTCGGAAATTGCCTGTAAATAGACCTAT9380.184243814671936No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9230.18129748501300313No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA9160.1799225311721678No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9140.1795296872176434No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG9060.17795831139954588No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8690.1706906982408448No Hit
CATTTAAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT8460.16617299276381436No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8440.16578014880928998No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8220.16145886530952175No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCGATTAATAATGTCCTCCTGT8000.15713758180975354No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7980.15674473785522916No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7970.15654831587796697No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7880.15478051808260723No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7810.15340556424177187No Hit
GATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGC7650.1502628126055768No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7550.14829859283295488No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7500.14731648294664396No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7420.1457451071285464No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7350.14437015328771105No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA7320.14378088735592448No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7300.1433880434014001No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7270.14279877746961353No Hit
CCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATG7200.14142382362877817No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6850.13454905442460147No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6780.1331741005837661No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6610.12983492697030885No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6360.12492437753875406No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTCCGAAAAC6360.12492437753875406No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC6340.12453153358422968No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6340.12453153358422968No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA6320.1241386896297053No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6240.12256731381160774No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT6130.12040667206172365No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6050.1188352962436261No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6050.1188352962436261No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6020.11824603031183954No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6020.11824603031183954No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5940.11667465449374201No Hit
TTATGAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA5930.11647823251647982No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA5930.11647823251647982No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5890.11569254460743103No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5840.11471043472112007No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5810.1141211687893335No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5800.1139247468120713No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5760.11313905890302253No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5760.11313905890302253No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5750.11294263692576034No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5730.11254979297123596No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5720.11235337099397377No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5700.11196052703944939No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA5680.111567683084925No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5670.11137126110766281No Hit
GTCTATTTACAGGCAATTTCCGAAAACATTGTTTGAGTTTTAATACAATA5630.11058557319861405No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC5600.10999630726682746No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5580.10960346331230308No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5560.1092106193577787No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5530.10862135342599212No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5430.1066571336533702No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5400.10606786772158364No Hit
ATGTTAATATGGGCCTAAAAATCAGACAGCTATTGTGGTTTCACATTTCC5300.10410364794896172No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5290.10390722597169952No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5290.10390722597169952No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5190.1019430061990776No Hit
GCACGGGACCATGCAAGACATGCACGATTCCTGCTCAAGGAACCTCTATG5180.10174658422181541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)