FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622939
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30010.4817486142302858No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT26280.42187116234494876No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG23000.36921753173264155No Hit
CTTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT21120.33903801174753867No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20170.32378772239336434No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG18100.2905581445374266No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT17300.2777157956075956No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16730.26856562199509104No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAAAGTATGTA15410.24737574626086983No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15330.24609151136788673No Hit
CTCTTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT13320.21382510968168633No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT12870.20660128840865638No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC12550.20146434883672398No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT11680.18749829437553275No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAAAGTATGTAAA11430.18348506033496056No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10840.1740138279992102No Hit
ATTCTGTGTTGGGGTGAATTGATGAATCTGGCCACCTGGGTGGGAAGTAA9930.15940565609152743No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9770.15683718630556123No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA9770.15683718630556123No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9770.15683718630556123No Hit
GTATACATGCATAGAGAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA9360.15025548247902282No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9350.15009495311739995No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9340.14993442375577704No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA8910.1430316612059929No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8880.14255007312112422No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8850.14206848503625555No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8840.14190795567463269No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8740.14030266205840378No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT8560.13741313354919182No Hit
CTTTTCTGCCGTCTGACTTTTTTCCTTCTATTCGGGATCTCCTTGACACC8460.13580783993296294No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG8360.13420254631673406No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8340.13388148759348828No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8330.13372095823186542No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7900.12681819568208122No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7870.12633660759721257No Hit
CTCTATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA7600.1220023148333946No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7390.11863119823931398No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7360.11814961015444529No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7280.1168653752614622No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7230.11606272845334775No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7160.11493902292198754No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7150.11477849356036467No Hit
GTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTC7020.11269161185926713No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7020.11269161185926713No Hit
CTATTCTGTGTTGGGGTGAATTGATGAATCTGGCCACCTGGGTGGGAAGT6970.11188896505115269No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC6940.11140737696628401No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA6880.1104442007965467No Hit
ATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATG6880.1104442007965467No Hit
GATTTGCCTCTGGCCAATGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA6870.1102836714349238No Hit
CAAGCACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAAT6850.10996261271167802No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6680.10723361356408895No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6670.10707308420246604No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA6640.1065914961175974No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6630.1064309667559745No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6630.1064309667559745No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG6420.10305985016189387No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6380.1024177327154023No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6340.10177561526891075No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6280.10081243909917344No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTCTCTATGCATGTATA6250.10033085101430476No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA6230.10000979229105898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)