FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420458
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACAGCATGTTCAGG15250.36269972268335954No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC15110.3593700203111845No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12590.2994353776120326No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12250.2913489575653216No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAGCCTTAT10710.25472223147139544No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10540.25067902144804No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10510.2499655137968596No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10430.24806282672704527No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG9540.2268954330753607No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT9370.2228522230520052No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9220.2192846847961033No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9220.2192846847961033No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9150.21761983361001574No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8700.20691721884231007No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8410.20001997821423304No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7950.18907952756280058No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7430.17671206160900732No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7390.17576071807410013No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7380.17552288219037337No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7380.17552288219037337No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7240.17219317981819823No Hit
ATGTAAGACCTTGGGCAAGACCTGTTGGGCGTTCACGGTGGTCTCCATGC7210.17147967216701787No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7170.1705283286321107No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7120.16933914921347673No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6960.16553377507384806No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6870.1633932521203069No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6830.16244190858539972No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6710.15958787798067822No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6710.15958787798067822No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6590.1567338473759567No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6560.1560203397247763No Hit
CTCCAAAAGACACCAGATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6370.15150145793396724No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6230.14817175556179216No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG6180.14698257614315818No Hit
CATTTGAACCCTAATAAAACCAAACGGTGGGGCTACTCCCTTAACTTCAT6140.14603123260825102No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5930.14103667904998834No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5890.14008533551508118No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACAGCA5860.1393718278639008No Hit
CTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACTGT5840.1388961560964472No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACCACATT5800.13794481256154004No Hit
GTTCAGGATAATAAGGCTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5680.1350907819568185No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5600.13318809488700417No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGACGGAAA5540.13176107958464342No Hit
GTGCACACGGTCCGGCGGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5530.13152324370091661No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5480.13033406428228264No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5480.13033406428228264No Hit
GGATAATAAGGCTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5420.1289070489799219No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5390.12819354132874153No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACAGCATGTTCA5290.12581518249147358No Hit
AACCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT5280.12557734660774678No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5270.12533951072402No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCGGATGAGAAGGCACAG5170.12296115188675207No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5080.12082062893321094No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5070.12058279304948413No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5070.12058279304948413No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT4980.11844227009594299No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGCGAGTTGATGAATCTG4950.11772876244476262No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4950.11772876244476262No Hit
GTCATGAAGACAGCCTACTCCCATCTCTCCACCTCTGAGAGACAGTCATC4940.11749092656103581No Hit
CTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACCACATTC4930.11725309067730905No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4920.11701525479358224No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4890.11630174714240187No Hit
TTCTCTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGGTGG4890.11630174714240187No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT4880.11606391125867507No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4850.1153504036074947No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4830.1148747318400411No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4820.1146368959563143No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4800.11416122418886072No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG4780.11368555242140713No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG4760.11320988065395354No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4730.11249637300277317No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4730.11249637300277317No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4730.11249637300277317No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4720.11225853711904638No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4710.11202070123531957No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4710.11202070123531957No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4640.11035585004923203No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTTGGGCGTTC4640.11035585004923203No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4630.11011801416550522No Hit
ATCCTGAACATGCTGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4590.10916667063059805No Hit
GTACAAAGATCATTAACCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTA4570.10869099886314448No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4550.10821532709569089No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4540.10797749121196411No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGCGAGTTGATGA4500.10702614767705693No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4470.10631264002587655No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4460.10607480414214976No Hit
CCAAAAGACACCAGATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4460.10607480414214976No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4430.10536129649096938No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4370.1039342811886086No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4360.10369644530488181No Hit
GATTAACAGCATGTTCAGGATAATAAGGCTTAATGCCTTTATCCAAGGGC4330.10298293765370144No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4310.10250726588624787No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4260.1013180864676139No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4240.10084241470016031No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4210.10012890704897992No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4210.10012890704897992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)