FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420458
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACAGCATGTTCAGG16830.40027779231219285No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC14410.3427215084503089No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12220.29063544991414125No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11720.2787436557278016No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10950.2604302926808385No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10590.25186820086667394No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10530.2504411855643132No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9840.23403050958716448No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAGCCTTAT9510.2261819254241803No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT9370.2228522230520052No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9360.2226143871682784No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9160.21785766949374255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9150.21761983361001574No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG8320.19787945526069192No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8240.1959767681908776No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8220.19550109642342398No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8010.1905065428651613No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7960.18931736344652736No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7700.18313363046963071No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7420.17647422572528054No Hit
CTCCAAAAGACACCAGATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7010.166722954492482No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6930.16482026742266764No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6670.15863653444577103No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6470.15387981677123516No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6460.15364198088750838No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6420.15269063735260122No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6410.1524528014688744No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6340.15078795028278688No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5950.14151235081744193No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG5830.1386583202127204No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5820.13842048432899362No Hit
CTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACTGT5780.13746914079408645No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5770.13723130491035967No Hit
ATGTAAGACCTTGGGCAAGACCTGTTGGGCGTTCACGGTGGTCTCCATGC5760.13699346902663287No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACAGCA5720.1360421254917257No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACCACATT5700.1355664537242721No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5660.13461511018936495No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5640.13413943842191134No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5620.13366376665445776No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5560.132236751352097No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5370.12771786956128792No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5330.12676652602638075No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5270.12533951072402No Hit
CATTTGAACCCTAATAAAACCAAACGGTGGGGCTACTCCCTTAACTTCAT5270.12533951072402No Hit
GGATAATAAGGCTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5270.12533951072402No Hit
GTTCAGGATAATAAGGCTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5260.1251016748402932No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5160.12272331600302529No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACAGCATGTTCA5140.12224764423557169No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCCGCCGGACCGTGTG5130.12200980835184488No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5010.11915577774712337No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4970.11820443421221621No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4970.11820443421221621No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4920.11701525479358224No Hit
CTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACCACATTC4900.11653958302612864No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4840.11511256772376789No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4840.11511256772376789No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4820.1146368959563143No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4810.11439906007258752No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC4790.11392338830513392No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4750.11297204477022675No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG4690.111545029467866No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTTGGGCGTTC4680.1113071935841392No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4670.1110693577004124No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4660.11083152181668562No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4640.11035585004923203No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4590.10916667063059805No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4580.10892883474687128No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4540.10797749121196411No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4510.1072639835607837No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGACGGAAA4470.10631264002587655No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT4470.10631264002587655No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG4420.10512346060724258No Hit
TTCTCTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGGTGG4420.10512346060724258No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4410.10488562472351579No Hit
GTACAAAGATCATTAACCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTA4380.10417211707233541No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4380.10417211707233541No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGCGAGTTGATGA4360.10369644530488181No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4340.10322077353742823No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4280.10179375823506746No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA4250.10108025058388709No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4240.10084241470016031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)