FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420073
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG25510.6072754021324865No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG23640.5627593299259891No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT23080.5494283136502465No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT22960.5465716673054445No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC13930.3316090298590959No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13800.3285143296522271No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13720.32660989875569246No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT12660.30137618937660837No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12390.29494873510080394No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC11650.2773327493078584No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA11590.2759044261354574No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10720.2551937401356431No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10390.24733796268743766No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10380.24709990882537083No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10090.24019634682543273No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9750.23210251551516048No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG9320.22186619944628674No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA9170.21829539151528424No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA8890.21162988337741295No Hit
TTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG8820.20996350634294517No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8410.20020329799820508No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA8370.19925108254993776No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA8320.1980608132396036No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8280.19710859779133627No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7970.1897289280672645No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7940.18901476648106402No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT7490.1783023426880566No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA7210.17163683455018533No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6880.16378105710197988No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT6690.1592580337227101No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6690.1592580337227101No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6670.15878192599857643No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6660.1585438721365096No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT6640.15806776441237594No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6330.15068809468830419No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6190.14735534061936853No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6150.1464031251711012No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6150.1464031251711012No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA6100.14521285586076704No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6100.14521285586076704No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGT6040.14378453268836605No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT6030.14354647882629926No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5990.1425942633780319No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5920.1409278863435641No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5900.14045177861943045No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTCTT5800.1380712399987621No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5770.13735707841256162No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5710.13592875524016065No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5650.13450043206775966No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5640.1342623782056928No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5620.13378627048155914No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA5600.1333101627574255No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5560.13235794730915817No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCAAGAAGATCTCGAA5430.12926324710228937No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5360.12759687006782156No Hit
TTCTTCTACTGTACCTCTTTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5340.12712076234368788No Hit
CATTTACAGGAGGATATTATTAATAGATGTCAACAATATGTGGGCCCTCT5260.12521633144715322No Hit
GGGTAAAGGTGCAGATATTGGTTACACAGAAAGGCCTTGTAAGTTGGCGA5230.12450216986095275No Hit
ATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA5140.12235968510235125No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5140.12235968510235125No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5040.11997914648168295No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4950.11783666172308145No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4870.11593223082654683No Hit
GCTTTATTCTTCTACTGTACCTCTTTTTAATCCTGAGTGGCAAACTCCCT4860.11569417696447998No Hit
GCTTTAGTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA4750.11307558448174485No Hit
GATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT4720.11236142289554434No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4690.11164726130934385No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4640.1104569919990097No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT4600.10950477655074238No Hit
CTTTACCACAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAA4570.10879061496454187No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4500.10712423793007406No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4460.10617202248180674No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4460.10617202248180674No Hit
CTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTAT4460.10617202248180674No Hit
ATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA4420.1052198070335394No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4410.10498175317147258No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTG4410.10498175317147258No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4400.10474369930940575No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA4380.10426759158527207No Hit
TCCCGATACAGAGCAGAGGCGGTGTCAAGAAGATCTCGAATAGAAGGAAA4330.10307732227493793No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4320.10283926841287108No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCT4320.10283926841287108No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4250.10117289137840327No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4240.10093483751633646No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4210.10022067593013595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)