FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420073
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG28210.671549944890531No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT21930.5220521195125609No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT21720.5170529884091575No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG21540.5127680188919544No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13990.3330373530314969No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13650.32494352172122465No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC13570.32303909082469No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13470.3206585522040217No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA12600.2999478662042074No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC12540.2985195430318064No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT11590.2759044261354574No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10570.2516229322046406No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10420.24805212427363815No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10080.2399582929633659No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9990.23781580820476442No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9720.23138835392895996No Hit
TTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG9270.22067593013595255No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA8760.20853518317054415No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA8610.2049643752395417No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8440.20091745958440557No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG8380.19948913641200458No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA7700.18330147379146006No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7630.18163509675699224No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7270.17306515772258632No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT6940.16520938027438087No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA6920.16473327255024722No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6850.1630668955157794No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA6770.16116246461924474No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6620.1575916566882423No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6610.15735360282617544No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA6550.15592527965377448No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA6430.1530686333089725No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6410.15259252558483882No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6300.1499739331021037No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6230.14830755606763585No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6220.14806950220556903No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6080.1447367481366334No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5990.1425942633780319No Hit
TTCTTCTACTGTACCTCTTTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5980.14235620951596506No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5810.13830929386082894No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5790.1378331861366953No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5770.13735707841256162No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT5760.1371190245504948No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTCTT5640.1342623782056928No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5620.13378627048155914No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5600.1333101627574255No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5550.13211989344709135No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5550.13211989344709135No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5540.1318818395850245No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT5530.13164378572295768No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5530.13164378572295768No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5410.1287871393781557No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT5370.12783492392988838No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5190.12354995441268542No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5030.11974109261961612No Hit
GCTTTATTCTTCTACTGTACCTCTTTTTAATCCTGAGTGGCAAACTCCCT4960.1180747155851483No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT4920.11712250013688097No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4910.11688444627481415No Hit
CATTTACAGGAGGATATTATTAATAGATGTCAACAATATGTGGGCCCTCT4900.11664639241274731No Hit
ATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA4870.11593223082654683No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGT4840.11521806924034632No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4750.11307558448174485No Hit
GATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT4740.112837530619678No Hit
GGGTAAAGGTGCAGATATTGGTTACACAGAAAGGCCTTGTAAGTTGGCGA4740.112837530619678No Hit
GCTTTAGTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA4680.11140920744727703No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG4660.11093309972314337No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCAAGAAGATCTCGAA4640.1104569919990097No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4590.10926672268867553No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT4520.10760034565420772No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4460.10617202248180674No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4460.10617202248180674No Hit
ATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA4450.10593396861973992No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4450.10593396861973992No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA4370.10402953772320525No Hit
CCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTG4360.10379148386113841No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4360.10379148386113841No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTG4330.10307732227493793No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4310.10260121455080426No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4280.10188705296460378No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4280.10188705296460378No Hit
CTTTACCACAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAA4260.10141094524047012No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4250.10117289137840327No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4230.10069678365426961No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4210.10022067593013595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)