FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459552
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG26370.5738197200752037No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20170.43890571687208413No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATGCCTGCTAGG16610.36143896664577674No Hit
ATTCCTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTT13930.30312130074507343No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13610.2961579973539447No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12480.27156883225402134No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAACGTAAGA11920.259383051319546No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11560.25154933500452614No Hit
TTATTAATAGATGTCAACAGTATGTGGGCCCTCTTACAGTTAATGAAAAA11270.24523884130631574No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10200.22195529559222898No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9580.20846389527191697No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTT9290.20215340157370654No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9230.20084778218786992No Hit
CTGTAAGAGGGCCCACATACTGTTGACATCTATTAATAATGTCCTCCTGT8550.18605076248172134No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCA8530.18561555601977578No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8150.1773466332428104No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7770.169077710465845No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7400.16102639091985238No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7310.15906796184109742No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTATAAGGCC7220.15710953276234244No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7130.15515110368358748No Hit
CTTTTACACAGTGCGGCTATCCCGCCTTAATGCCTTTATATGCATGTATA7090.15428069075969641No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAAC7070.15384548429775086No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7010.1525398649119142No Hit
GTATACATGCATATAAAGGCATTAAGGCGGGATAGCCGCACTGTGTAAAA6650.14470614859689437No Hit
GTGCTGGTGAACGGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6610.14383573567300328No Hit
GTATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTA6540.14231251305619386No Hit
TAGCCGCACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6520.14187730659424833No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6470.14078929043938446No Hit
GGATAAAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCATTAAC6390.13904846459160225No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6280.13665482905090173No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6270.13643722581992898No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6270.13643722581992898No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC6230.1355668128960379No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6190.13469639997214677No Hit
TCTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6110.1329555741243646No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT6030.13121474827658242No Hit
TCTGCGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5960.129691525659773No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT5820.12664508042615416No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5670.12338103196156258No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5590.12164020611378037No Hit
CCCACATACTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTT5440.11837615764918878No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA5270.11467690272265163No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5220.11358888656778775No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5180.11271847364389667No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5120.11141285425806002No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG5050.10988963164125061No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5030.10945442517930506No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5030.10945442517930506No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4870.10597277348374069No Hit
CTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4840.10531996379082237No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA4810.10466715409790406No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4650.10118550240233966No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4640.1009678991713669No Hit
TGTCAACAGTATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAA4610.10031508947844858No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4600.1000974862474758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)