FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459552
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG30660.6671715061625235No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20090.43716489102430195No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTATGCCTGCTAGG16180.3520820277139475No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13230.2878890745769793No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAACGTAAGA12600.27418007102569464No Hit
ATTCCTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTT12520.2724392451779124No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12290.2674343708655386No Hit
TTATTAATAGATGTCAACAGTATGTGGGCCCTCTTACAGTTAATGAAAAA11900.2589478448576005No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10720.23327066360281315No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10120.22021446974444678No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10050.21869124712763735No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTT9660.21020472111969918No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9490.20650546619316204No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCA9340.20324141772857046No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8540.18583315925074856No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8190.17821704616670148No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8120.17669382354989208No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7290.15863275537915186No Hit
CTGTAAGAGGGCCCACATACTGTTGACATCTATTAATAATGTCCTCCTGT7290.15863275537915186No Hit
CTTTTACACAGTGCGGCTATCCCGCCTTAATGCCTTTATATGCATGTATA7190.15645672306942413No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7180.15623911983845137No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTATAAGGCC6960.15145184875705034No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6720.14622937121370377No Hit
GTGCTGGTGAACGGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6690.14557656152078546No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6510.14165970336327555No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6510.14165970336327555No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6500.14144210013230277No Hit
GTATACATGCATATAAAGGCATTAAGGCGGGATAGCCGCACTGTGTAAAA6220.1353492096650651No Hit
GTATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAGTTAATTA6200.13491400320311955No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC6160.13404359027922846No Hit
TCTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6110.1329555741243646No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5820.12664508042615416No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5820.12664508042615416No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAAC5700.12403384165448089No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5680.12359863519253533No Hit
GGATAAAACCTAGCAGGCATAATTAACTTTAATCTCCTTTTTTCATTAAC5630.12251061903767148No Hit
TCTGCGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5620.12229301580669868No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA5520.12011698349697096No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5520.12011698349697096No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5320.1157649188775155No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5320.1157649188775155No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5320.1157649188775155No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5250.11424169626070607No Hit
CTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5220.11358888656778775No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5150.11206566395097835No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4940.10749599610055009No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4910.10684318640763178No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4850.10553756702179513No Hit
TAGCCGCACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4800.10444955086693128No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT4780.10401434440498573No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC4730.10292632825012185No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGCACCTTTACCCCGTTGCC4690.10205591532623076No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4610.10031508947844858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)