FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408337
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCAAACTGAGCCATGAGAAACGGGCTGAGGCCCATTCCCATAGGAGT18080.4427715342964267No Hit
GTACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG17570.43028185053032175No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16640.40750654483918924No Hit
GTTTGCTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT16010.3920781119516478No Hit
CTAAAAAACTGAGCCATGAGAAACGGGCTGAGGCCCATTCCCATAGGAGT14680.359506975855727No Hit
CCTCCCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14410.352894790332495No Hit
CTATCAATAATGTCCTCTTGTAAATGAATATGAGGAAAGGAGGGAGTTTG13830.3386908362455521No Hit
GTTTTTTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT13560.33207865072232007No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11420.27967095805670317No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10670.26130377604772526No Hit
ATCCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACG10390.25444669476437354No Hit
GTATACAGGCATATAAAGGCATCAGAGCAGGATAGCCACATTGCGTAAAA10150.24856919652150064No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG9850.24122232371790947No Hit
CTATCGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAG9370.22946732723216362No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9260.2267734738708469No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9260.2267734738708469No Hit
CCTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8760.21452868586486162No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG8500.2081613961017493No Hit
TGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCG8360.2047328554600734No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8240.20179410633863695No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7970.19518192081540492No Hit
CTATTAATAATGTCCTCTTGTAAATGAATATGAGGAAAGGAGGGAGTTTG7960.1949370250552852No Hit
ATTATGATTCAAATGTATACCCAAAGATAAAAGAAAATTGGTAACAGCGG7920.1939574420148064No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC7880.19297785897432757No Hit
GGATCATATTATACAAAAAATCAAGCAATGTTTTCGTAAACTGCCTGTAA7660.18759015225169406No Hit
GGATAGCCACATTGCGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7490.1834269243296591No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7370.18048817520822263No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7360.18024327944810292No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7270.17803921760702557No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7200.17632494728618764No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7190.17608005152606793No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG6930.16971276176295558No Hit
GTCTATTTACAGGCAGTTTACGAAAACATTGCTTGATTTTTTGTATAATA6750.1653046380808009No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC6490.15893734831768858No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6420.15722307799685065No Hit
ATCCTGCTCTGATGCCTTTATATGCCTGTATACAATCTAAGCAGGCTTTC6380.15624349495637183No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6300.15428432887541418No Hit
TTATATGCCTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA6290.15403943311529447No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6260.15330474583493536No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTATC6250.15305985007481565No Hit
ATCTCAATGTCAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGC6200.15183537127421715No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6180.15134557975397772No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG6120.1498762051932595No Hit
CTTTACCACAGGATCATATTATACAAAAAATCAAGCAATGTTTTCGTAAA6120.1498762051932595No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6100.14938641367302008No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA6050.14816193487242155No Hit
ATTTACAGGCAGTTTACGAAAACATTGCTTGATTTTTTGTATAATATGAT6010.14718235183194273No Hit
GTTTTTTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT5910.1447333942307457No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG5820.14252933238966833No Hit
ACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAACTGTAAGA5700.13959058326823187No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGC5670.13885589598787276No Hit
ACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAACTGTAAGA5660.13861100022775308No Hit
ACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGG5440.13322329350511952No Hit
GTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT5300.1297947528634437No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGT5260.12881516982296484No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGC5210.1275906910223663No Hit
CTGCAAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGC5190.12710089950212694No Hit
GTCCCGTACTGGTGGTTGATGTTCATTTAGATTCCCGAGATTGAGATCTT5160.1263662122217678No Hit
GTTATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5140.12587642070152838No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5110.12514173342116927No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5060.12391725462057077No Hit
GGCATATAAAGGCATCAGAGCAGGATAGCCACATTGCGTAAAAGGGGCAG5000.12244788005985252No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5000.12244788005985252No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4950.121223401259254No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4920.12048871397889488No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4900.11999892245865547No Hit
GGGTTATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATAC4870.11926423517829635No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAAGAGTCCTCTTATGT4840.11852954789793725No Hit
GCCCCACCGTTTGGTTTTATTAGGATTCAAATGTATACCCAAAGATAAAA4830.11828465213781754No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA4830.11828465213781754No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4790.11730506909733872No Hit
GTACAGACTTGGCCCCCAATACCACATCATCAACATAACTGAAAGCCAAA4790.11730506909733872No Hit
GCTTAGATTGTATACAGGCATATAAAGGCATCAGAGCAGGATAGCCACAT4760.1165703818169796No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4690.11485611149614167No Hit
GAGCAGGATAGCCACATTGCGTAAAAGGGGCAGCAAAGCCCAAAAGACCC4670.11436631997590226No Hit
GGCCTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG4650.11387652845566285No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4620.11314184117530374No Hit
ATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTT4510.11044798781398699No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4490.10995819629374758No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4460.10922350901338845No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4430.10848882173302933No Hit
GTGGCTATCCTGCTCTGATGCCTTTATATGCCTGTATACAATCTAAGCAG4380.10726434293243081No Hit
GGTTAGGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4360.1067745514121914No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4320.10579496837171258No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4290.10506028109135347No Hit
ATATTATACAAAAAATCAAGCAATGTTTTCGTAAACTGCCTGTAAATAGA4290.10506028109135347No Hit
GGTCTATTTACAGGCAGTTTACGAAAACATTGCTTGATTTTTTGTATAAT4280.10481538533123377No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCAAGG4250.10408069805087465No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4150.1016317404496776No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4110.10065215740919878No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAAC4100.10040726164907907No Hit
CTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGC4100.10040726164907907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)